» Articles » PMID: 25329171

COPASutils: an R Package for Reading, Processing, and Visualizing Data from COPAS Large-particle Flow Cytometers

Overview
Journal PLoS One
Date 2014 Oct 21
PMID 25329171
Citations 29
Authors
Affiliations
Soon will be listed here.
Abstract

The R package COPASutils provides a logical workflow for the reading, processing, and visualization of data obtained from the Union Biometrica Complex Object Parametric Analyzer and Sorter (COPAS) or the BioSorter large-particle flow cytometers. Data obtained from these powerful experimental platforms can be unwieldy, leading to difficulties in the ability to process and visualize the data using existing tools. Researchers studying small organisms, such as Caenorhabditis elegans, Anopheles gambiae, and Danio rerio, and using these devices will benefit from this streamlined and extensible R package. COPASutils offers a powerful suite of functions for the rapid processing and analysis of large high-throughput screening data sets.

Citing Articles

Naturally occurring variation in a cytochrome P450 modifies thiabendazole responses independently of beta-tubulin.

Collins J, Dilks C, Hahnel S, Rodriguez B, Fox B, Redman E PLoS Pathog. 2025; 21(1):e1012602.

PMID: 39808673 PMC: 11771912. DOI: 10.1371/journal.ppat.1012602.


Machine learning-based analysis of microfluidic device immobilized C. elegans for automated developmental toxicity testing.

DuPlissis A, Medewar A, Hegarty E, Laing A, Shen A, Gomez S Sci Rep. 2025; 15(1):15.

PMID: 39747450 PMC: 11696900. DOI: 10.1038/s41598-024-84842-x.


Divergent toxin alleles are suppressed by distinct mechanisms.

Zdraljevic S, Walter-McNeill L, Bruni G, Bloom J, Leighton D, Collins J bioRxiv. 2024; .

PMID: 39605437 PMC: 11601442. DOI: 10.1101/2024.04.26.591160.


vivoBodySeg: Machine learning-based analysis of C. elegans immobilized in vivoChip for automated developmental toxicity testing.

DuPlissis A, Medewar A, Hegarty E, Laing A, Shen A, Gomez S Res Sq. 2024; .

PMID: 39281859 PMC: 11398583. DOI: 10.21203/rs.3.rs-4796642/v1.


Praziquantel inhibits Caenorhabditis elegans development and species-wide differences might be cct-8-dependent.

Wit J, Dilks C, Zhang G, Kim Guisbert K, Zdraljevic S, Guisbert E PLoS One. 2023; 18(8):e0286473.

PMID: 37561720 PMC: 10414639. DOI: 10.1371/journal.pone.0286473.


References
1.
Boyd W, Smith M, Kissling G, Freedman J . Medium- and high-throughput screening of neurotoxicants using C. elegans. Neurotoxicol Teratol. 2009; 32(1):68-73. PMC: 2819467. DOI: 10.1016/j.ntt.2008.12.004. View

2.
Giacomotto J, Segalat L . High-throughput screening and small animal models, where are we?. Br J Pharmacol. 2010; 160(2):204-16. PMC: 2874843. DOI: 10.1111/j.1476-5381.2010.00725.x. View

3.
Morton E, Lamitina T . A suite of MATLAB-based computational tools for automated analysis of COPAS Biosort data. Biotechniques. 2010; 48(6):xxv-xxx. PMC: 2892394. DOI: 10.2144/000113427. View

4.
Veneman W, Stockhammer O, de Boer L, Zaat S, Meijer A, Spaink H . A zebrafish high throughput screening system used for Staphylococcus epidermidis infection marker discovery. BMC Genomics. 2013; 14:255. PMC: 3638012. DOI: 10.1186/1471-2164-14-255. View

5.
Boyd W, McBride S, Rice J, Snyder D, Freedman J . A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay. Toxicol Appl Pharmacol. 2010; 245(2):153-9. PMC: 2871981. DOI: 10.1016/j.taap.2010.02.014. View