Detection and Validation of Stay-green QTL in Post-rainy Sorghum Involving Widely Adapted Cultivar, M35-1 and a Popular Stay-green Genotype B35

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2014 Oct 19

PMID 25326366

Citations 30

Authors

Nagaraja Reddy Rama Reddy

Madhusudhana Ragimasalawada

Murali Mohan Sabbavarapu

Seetharama Nadoor

Jagannatha Vishnu Patil

Affiliations

Soon will be listed here.

Abstract

Background: Sorghum [Sorghum bicolor (L.) Moench] is an important dry-land cereal of the world providing food, fodder, feed and fuel. Stay-green (delayed-leaf senescence) is a key attribute in sorghum determining its adaptation to terminal drought stress. The objective of this study was to validate sorghum stay-green quantitative trait loci (QTL) identified in the past, and to identify new QTL in the genetic background of a post-rainy adapted genotype M35-1.

Results: A genetic linkage map based on 245 F9 Recombinant Inbred Lines (RILs) derived from a cross between M35-1 (more senescent) and B35 (less senescent) with 237 markers consisting of 174 genomic, 60 genic and 3 morphological markers was used. The phenotypic data collected for three consecutive post-rainy crop seasons on the RIL population (M35-1 × B35) was used for QTL analysis. Sixty-one QTL were identified for various measures of stay-green trait and each trait was controlled by one to ten QTL. The phenotypic variation explained by each QTL ranged from 3.8 to 18.7%. Co-localization of QTL for more than five traits was observed on two linkage groups i.e. on SBI-09-3 flanked by S18 and Xgap206 markers and, on SBI-03 flanked by XnhsbSFCILP67 and Xtxp31. QTL identified in this study were stable across environments and corresponded to sorghum stay-green and grain yield QTL reported previously. Of the 60 genic SSRs mapped, 14 were closely linked with QTL for ten traits. A genic marker, XnhsbSFCILP67 (Sb03g028240) encoding Indole-3-acetic acid-amido synthetase GH3.5, was co-located with QTL for GLB, GLM, PGLM and GLAM on SBI-03. Genes underlying key enzymes of chlorophyll metabolism were also found in the stay-green QTL regions.

Conclusions: We validated important stay-green QTL reported in the past in sorghum and detected new QTL influencing the stay-green related traits consistently. Stg2, Stg3 and StgB were prominent in their expression. Collectively, the QTL/markers identified are likely candidates for subsequent verification for their involvement in stay-green phenotype using NILs and to develop drought tolerant sorghum varieties through marker-assisted breeding for terminal drought tolerance in sorghum.

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References

Srinivas G, Satish K, Madhusudhana R, Nagaraja Reddy R, Mohan S, Seetharama N . Identification of quantitative trait loci for agronomically important traits and their association with genic-microsatellite markers in sorghum. Theor Appl Genet. 2009; 118(8):1439-54. DOI: 10.1007/s00122-009-0993-6. View

Armstrong G, Apel K, Rudiger W . Does a light-harvesting protochlorophyllide a/b-binding protein complex exist?. Trends Plant Sci. 2000; 5(1):40-4. DOI: 10.1016/s1360-1385(99)01513-7. View

Christ B, Schelbert S, Aubry S, Sussenbacher I, Muller T, Krautler B . MES16, a member of the methylesterase protein family, specifically demethylates fluorescent chlorophyll catabolites during chlorophyll breakdown in Arabidopsis. Plant Physiol. 2011; 158(2):628-41. PMC: 3271755. DOI: 10.1104/pp.111.188870. View

Vadez V, Deshpande S, Kholova J, Hammer G, Borrell A, Talwar H . Stay-green quantitative trait loci's effects on water extraction, transpiration efficiency and seed yield depend on recipient parent background. Funct Plant Biol. 2020; 38(7):553-566. DOI: 10.1071/FP11073. View

Kusaba M, Tanaka A, Tanaka R . Stay-green plants: what do they tell us about the molecular mechanism of leaf senescence. Photosynth Res. 2013; 117(1-3):221-34. DOI: 10.1007/s11120-013-9862-x. View

Brown P, Klein P, Bortiri E, Acharya C, Rooney W, Kresovich S . Inheritance of inflorescence architecture in sorghum. Theor Appl Genet. 2006; 113(5):931-42. DOI: 10.1007/s00122-006-0352-9. View

Sato Y, Morita R, Katsuma S, Nishimura M, Tanaka A, Kusaba M . Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. Plant J. 2008; 57(1):120-31. DOI: 10.1111/j.1365-313X.2008.03670.x. View

Thomas H, Howarth C . Five ways to stay green. J Exp Bot. 2000; 51 Spec No:329-37. DOI: 10.1093/jexbot/51.suppl_1.329. View

Jiang G, He Y, Xu C, Li X, Zhang Q . The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross. Theor Appl Genet. 2003; 108(4):688-98. DOI: 10.1007/s00122-003-1465-z. View

10.

Green B, Pichersky E, Kloppstech K . Chlorophyll a/b-binding proteins: an extended family. Trends Biochem Sci. 1991; 16(5):181-6. DOI: 10.1016/0968-0004(91)90072-4. View

11.

Mace E, Singh V, Van Oosterom E, Hammer G, Hunt C, Jordan D . QTL for nodal root angle in sorghum (Sorghum bicolor L. Moench) co-locate with QTL for traits associated with drought adaptation. Theor Appl Genet. 2011; 124(1):97-109. DOI: 10.1007/s00122-011-1690-9. View

12.

Adams J, KELSO R, Cooley L . The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 1999; 10(1):17-24. DOI: 10.1016/s0962-8924(99)01673-6. View

13.

Mace E, Jordan D . Integrating sorghum whole genome sequence information with a compendium of sorghum QTL studies reveals uneven distribution of QTL and of gene-rich regions with significant implications for crop improvement. Theor Appl Genet. 2011; 123(1):169-91. DOI: 10.1007/s00122-011-1575-y. View

14.

De Simone V, Soccio M, Borrelli G, Pastore D, Trono D . Stay-green trait-antioxidant status interrelationship in durum wheat (Triticum durum) flag leaf during post-flowering. J Plant Res. 2013; 127(1):159-71. DOI: 10.1007/s10265-013-0584-0. View

15.

Hortensteiner S . Update on the biochemistry of chlorophyll breakdown. Plant Mol Biol. 2012; 82(6):505-17. DOI: 10.1007/s11103-012-9940-z. View

16.

Hui Z, Tian F, Wang G, Wang G, Wang W . The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1. Plant Cell Rep. 2012; 31(6):1073-84. DOI: 10.1007/s00299-012-1226-z. View

17.

Feltus F, Hart G, Schertz K, Casa A, Kresovich S, Abraham S . Alignment of genetic maps and QTLs between inter- and intra-specific sorghum populations. Theor Appl Genet. 2006; 112(7):1295-305. DOI: 10.1007/s00122-006-0232-3. View

18.

Sanchez A, Subudhi P, Rosenow D, Nguyen H . Mapping QTLs associated with drought resistance in sorghum (Sorghum bicolor L. Moench). Plant Mol Biol. 2002; 48(5-6):713-26. DOI: 10.1023/a:1014894130270. View

19.

Edwards G, Franceschi V, Voznesenskaya E . Single-cell C(4) photosynthesis versus the dual-cell (Kranz) paradigm. Annu Rev Plant Biol. 2004; 55:173-96. DOI: 10.1146/annurev.arplant.55.031903.141725. View

20.

Luquez V, Guiamet J . The stay green mutations d1 and d2 increase water stress susceptibility in soybeans. J Exp Bot. 2002; 53(373):1421-8. DOI: 10.1093/jexbot/53.373.1421. View