Methods for Gene Transfer to the Central Nervous System

Overview

Journal Adv Genet

Specialty Genetics

Date 2014 Oct 15

PMID 25311922

Citations 50

Authors

Boris Kantor

Rachel M Bailey

Keon Wimberly

Sahana N Kalburgi

Steven J Gray

Affiliations

Soon will be listed here.

Abstract

Gene transfer is an increasingly utilized approach for research and clinical applications involving the central nervous system (CNS). Vectors for gene transfer can be as simple as an unmodified plasmid, but more commonly involve complex modifications to viruses to make them suitable gene delivery vehicles. This chapter will explain how tools for CNS gene transfer have been derived from naturally occurring viruses. The current capabilities of plasmid, retroviral, adeno-associated virus, adenovirus, and herpes simplex virus vectors for CNS gene delivery will be described. These include both focal and global CNS gene transfer strategies, with short- or long-term gene expression. As is described in this chapter, an important aspect of any vector is the cis-acting regulatory elements incorporated into the vector genome that control when, where, and how the transgene is expressed.

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