Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions

Overview

Journal Endocrinol Metab (Seoul)

Specialty Endocrinology

Date 2014 Oct 14

PMID 25309794

Citations 2

Authors

Eun Soo Lee

Woon Ryoung Kim

Younghwa Kim

Hyun Woo Lee

Hyun Kim

Woong Sun

Affiliations

Soon will be listed here.

Abstract

Background: Adult neural stem cells have the potential for self-renewal and differentiation into multiple cell lineages via symmetric or asymmetric cell division. Preso1 is a recently identified protein involved in the formation of dendritic spines and the promotion of axonal growth in developing neurons. Preso1 can also bind to cell polarity proteins, suggesting a potential role for Preso1 in asymmetric cell division.

Methods: To investigate the distribution of Preso1, we performed immunohistochemistry with adult mouse brain slice. Also, polarized distribution of Preso1 was assessed by immunocytochemistry in cultured neural stem cells.

Results: Immunoreactivity for Preso1 (Preso1-IR) was strong in the rostral migratory stream and subventricular zone, where proliferating transit-amplifying cells and neuroblasts are prevalent. In cultured neural stem cells, Preso1-IR was unequally distributed in the cell cytosol. We also observed the distribution of Preso1 in the subgranular zone of the hippocampal dentate gyrus, another neurogenic region in the adult brain. Interestingly, Preso1-IR was transiently observed in the nuclei of doublecortin-expressing neuroblasts immediately after asymmetric cell division.

Conclusion: Our study demonstrated that Preso1 is asymmetrically distributed in the cytosol and nuclei of neural stem/progenitor cells in the adult brain, and may play a significant role in cell differentiation via association with cell polarity machinery.

Citing Articles

FERM domain-containing proteins are active components of the cell nucleus.

Borkuti P, Kristo I, Szabo A, Kovacs Z, Vilmos P Life Sci Alliance. 2024; 7(4).

PMID: 38296350 PMC: 10830384. DOI: 10.26508/lsa.202302489.

Articles in 'endocrinology and metabolism' in 2014.

Lee W Endocrinol Metab (Seoul). 2015; 30(1):47-52.

PMID: 25827457 PMC: 4384668. DOI: 10.3803/EnM.2015.30.1.47.

References

Legg J, Isacke C . Identification and functional analysis of the ezrin-binding site in the hyaluronan receptor, CD44. Curr Biol. 1998; 8(12):705-8. DOI: 10.1016/s0960-9822(98)70277-5. View

Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao B, Dembowy J . TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Nat Cell Biol. 2008; 10(7):837-48. DOI: 10.1038/ncb1748. View

Pan Z, Shang Y, Jia M, Zhang L, Xia C, Zhang M . Structural and biochemical characterization of the interaction between LGN and Frmpd1. J Mol Biol. 2013; 425(6):1039-49. DOI: 10.1016/j.jmb.2013.01.003. View

Moon Y, Kim J, Kim W, Kim H, Jang M, Nam Y . Function of ezrin-radixin-moesin proteins in migration of subventricular zone-derived neuroblasts following traumatic brain injury. Stem Cells. 2013; 31(8):1696-705. DOI: 10.1002/stem.1420. View

Oishi K, Sato N, Sagara J, Kawai A, Tsukita S . ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons. J Cell Biol. 1994; 126(2):391-401. PMC: 2200023. DOI: 10.1083/jcb.126.2.391. View

Murakami M, Tominaga J, Makita R, Uchijima Y, Kurihara Y, Nakagawa O . Transcriptional activity of Pax3 is co-activated by TAZ. Biochem Biophys Res Commun. 2005; 339(2):533-9. DOI: 10.1016/j.bbrc.2005.10.214. View

Tsukita S, Yonemura S . Cortical actin organization: lessons from ERM (ezrin/radixin/moesin) proteins. J Biol Chem. 1999; 274(49):34507-10. DOI: 10.1074/jbc.274.49.34507. View

Kanai F, Marignani P, Sarbassova D, Yagi R, Hall R, Donowitz M . TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins. EMBO J. 2000; 19(24):6778-91. PMC: 305881. DOI: 10.1093/emboj/19.24.6778. View

Liu C, Huang W, Lei Q . Regulation and function of the TAZ transcription co-activator. Int J Biochem Mol Biol. 2011; 2(3):247-56. PMC: 3193294. View

10.

Batchelor C, Woodward A, Crouch D . Nuclear ERM (ezrin, radixin, moesin) proteins: regulation by cell density and nuclear import. Exp Cell Res. 2004; 296(2):208-22. DOI: 10.1016/j.yexcr.2004.02.010. View

11.

Gee S, Milgram S, Kramer K, Conlon F, Moody S . Yes-associated protein 65 (YAP) expands neural progenitors and regulates Pax3 expression in the neural plate border zone. PLoS One. 2011; 6(6):e20309. PMC: 3110623. DOI: 10.1371/journal.pone.0020309. View

12.

Stenzel N, Fetzer C, Heumann R, Erdmann K . PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J Cell Sci. 2009; 122(Pt 18):3374-84. DOI: 10.1242/jcs.046854. View

13.

Lee H, Choi J, Shin H, Kim K, Yang J, Na M . Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J Neurosci. 2009; 28(53):14546-56. PMC: 6671232. DOI: 10.1523/JNEUROSCI.3112-08.2008. View

14.

Mo J, Lee D, Hong S, Han S, Yeo H, Sun W . Preso regulation of dendritic outgrowth through PI(4,5)P2-dependent PDZ interaction with βPix. Eur J Neurosci. 2012; 36(1):1960-70. DOI: 10.1111/j.1460-9568.2012.08124.x. View

15.

Schwamborn J, Berezikov E, Knoblich J . The TRIM-NHL protein TRIM32 activates microRNAs and prevents self-renewal in mouse neural progenitors. Cell. 2009; 136(5):913-25. PMC: 2988196. DOI: 10.1016/j.cell.2008.12.024. View

16.

Kim W, Chun S, Kim T, Kim H, Ono K, Takebayashi H . Evidence for the spontaneous production but massive programmed cell death of new neurons in the subcallosal zone of the postnatal mouse brain. Eur J Neurosci. 2011; 33(4):599-611. DOI: 10.1111/j.1460-9568.2010.07557.x. View

17.

Cui C, Cooper L, Yang X, Karsenty G, Aukhil I . Transcriptional coactivation of bone-specific transcription factor Cbfa1 by TAZ. Mol Cell Biol. 2003; 23(3):1004-13. PMC: 140696. DOI: 10.1128/MCB.23.3.1004-1013.2003. View

18.

Sudol M, Bork P, Einbond A, Kastury K, Druck T, Negrini M . Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain. J Biol Chem. 1995; 270(24):14733-41. DOI: 10.1074/jbc.270.24.14733. View

19.

Kim S . Polarized signaling: basolateral receptor localization in epithelial cells by PDZ-containing proteins. Curr Opin Cell Biol. 1998; 9(6):853-9. DOI: 10.1016/s0955-0674(97)80088-9. View

20.

Hu J, Yang L, Kammermeier P, Moore C, Brakeman P, Tu J . Preso1 dynamically regulates group I metabotropic glutamate receptors. Nat Neurosci. 2012; 15(6):836-44. PMC: 3434267. DOI: 10.1038/nn.3103. View