Effect of Acute and Chronic Electroconvulsive Shock on 5-hydroxytrypamine 6 Receptor Immunoreactivity in Rat Hippocampus

Overview

Journal Exp Neurobiol

Specialty Neurology

Date 2014 Sep 27

PMID 25258570

Authors

Hyun Jung Kim

Seungwoo Kang

Hyun Ju Kim

Sun-Hye Choi

Seungkeun Shin

Hyung Ha Lee

Hyewhon Rhim

Kyung Ho Shin

Affiliations

Soon will be listed here.

Abstract

Electroconvulsive shock (ECS) induces not only an antidepressant effect but also adverse effects such as amnesia. One potential mechanism underlying both the antidepressant and amnesia effect of ECS may involve the regulation of serotonin (5-hydroxytryptamine) 6 (5-HT6) receptor, but less is known about the effects of acute ECS on the changes in 5-HT6 receptor expression in the hippocampus. In addition, as regulation of 5-HT receptor expression is influenced by the number of ECS treatment and by interval between ECS treatment and sacrifice, it is probable that magnitude and time-dependent changes in 5-HT6 receptor expression could be influenced by repeated ECS exposure. To explore this possibility, we observed and compared the changes of 5-HT6 receptor immunoreactivity (5-HT6 IR) in rat hippocampus at 1, 8, 24, or 72 h after the treatment with either a single ECS (acute ECS) or daily ECS for 10 days (chronic ECS). We found that acute ECS increased 5-HT6 IR in the CA1, CA3, and granule cell layer of hippocampus, reaching peak levels at 8 h and returning to basal levels 72 h later. The magnitude and time-dependent changes in 5-HT6 IR observed after acute ECS were not affected by chronic ECS. These results demonstrate that both acute and chronic ECS transiently increase the 5-HT6 IR in rat hippocampus, and suggest that the magnitude and time-dependent changes in 5-HT6 IR in the hippocampus appear not to be influenced by repeated ECS treatment.

References

Burnet P, Sharp T, LeCorre S, Harrison P . Expression of 5-HT receptors and the 5-HT transporter in rat brain after electroconvulsive shock. Neurosci Lett. 2000; 277(2):79-82. DOI: 10.1016/s0304-3940(99)00857-5. View

Li B, Suemaru K, Cui R, Araki H . Repeated electroconvulsive stimuli have long-lasting effects on hippocampal BDNF and decrease immobility time in the rat forced swim test. Life Sci. 2007; 80(16):1539-43. DOI: 10.1016/j.lfs.2007.01.032. View

Marcos B, Cabero M, Solas M, Aisa B, Ramirez M . Signalling pathways associated with 5-HT6 receptors: relevance for cognitive effects. Int J Neuropsychopharmacol. 2009; 13(6):775-84. DOI: 10.1017/S146114570999054X. View

Marazziti D, Baroni S, Pirone A, Giannaccini G, Betti L, Testa G . Serotonin receptor of type 6 (5-HT6) in human prefrontal cortex and hippocampus post-mortem: an immunohistochemical and immunofluorescence study. Neurochem Int. 2012; 62(2):182-8. DOI: 10.1016/j.neuint.2012.11.013. View

Squire L . Memory functions as affected by electroconvulsive therapy. Ann N Y Acad Sci. 1986; 462:307-14. DOI: 10.1111/j.1749-6632.1986.tb51265.x. View

Jun H, Hussaini S, Rigby M, Jang M . Functional role of adult hippocampal neurogenesis as a therapeutic strategy for mental disorders. Neural Plast. 2013; 2012:854285. PMC: 3549353. DOI: 10.1155/2012/854285. View

Masi G, Brovedani P . The hippocampus, neurotrophic factors and depression: possible implications for the pharmacotherapy of depression. CNS Drugs. 2011; 25(11):913-31. DOI: 10.2165/11595900-000000000-00000. View

Marek G . Regulation of rat cortical 5-hydroxytryptamine2A receptor-mediated electrophysiological responses by repeated daily treatment with electroconvulsive shock or imipramine. Eur Neuropsychopharmacol. 2008; 18(7):498-507. PMC: 2453314. DOI: 10.1016/j.euroneuro.2008.01.003. View

Carr G, Schechter L, Lucki I . Antidepressant and anxiolytic effects of selective 5-HT6 receptor agonists in rats. Psychopharmacology (Berl). 2010; 213(2-3):499-507. PMC: 2910165. DOI: 10.1007/s00213-010-1798-7. View

10.

Lerer B, Stanley M, McIntyre I, ALTMAN H . Electroconvulsive shock and brain muscarinic receptors: relationship to anterograde amnesia. Life Sci. 1984; 35(26):2659-64. DOI: 10.1016/0024-3205(84)90035-3. View

11.

Yun H, Kim S, Kim H, Kostenis E, Kim J, Seong J . The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. J Biol Chem. 2006; 282(8):5496-505. DOI: 10.1074/jbc.M606215200. View

12.

Svenningsson P, Tzavara E, Qi H, Carruthers R, Witkin J, Nomikos G . Biochemical and behavioral evidence for antidepressant-like effects of 5-HT6 receptor stimulation. J Neurosci. 2007; 27(15):4201-9. PMC: 6672541. DOI: 10.1523/JNEUROSCI.3110-06.2007. View

13.

Foley A, Murphy K, Hirst W, Gallagher H, Hagan J, Upton N . The 5-HT(6) receptor antagonist SB-271046 reverses scopolamine-disrupted consolidation of a passive avoidance task and ameliorates spatial task deficits in aged rats. Neuropsychopharmacology. 2003; 29(1):93-100. DOI: 10.1038/sj.npp.1300332. View

14.

Jacobsen J, Mork A . The effect of escitalopram, desipramine, electroconvulsive seizures and lithium on brain-derived neurotrophic factor mRNA and protein expression in the rat brain and the correlation to 5-HT and 5-HIAA levels. Brain Res. 2004; 1024(1-2):183-92. DOI: 10.1016/j.brainres.2004.07.065. View

15.

Gluck M, Myers C . Psychobiological models of hippocampal function in learning and memory. Annu Rev Psychol. 1997; 48:481-514. DOI: 10.1146/annurev.psych.48.1.481. View

16.

Nikiforuk A, Kos T, Wesolowska A . The 5-HT6 receptor agonist EMD 386088 produces antidepressant and anxiolytic effects in rats after intrahippocampal administration. Psychopharmacology (Berl). 2011; 217(3):411-8. DOI: 10.1007/s00213-011-2297-1. View

17.

Thiagarajan A, Gleiter C, Mefford I, Eskay R, Nutt D . Effect of single and repeated electroconvulsive shock on the hypothalamic-pituitary-adrenal axis and plasma catecholamines in rats. Psychopharmacology (Berl). 1989; 97(4):548-52. DOI: 10.1007/BF00439562. View

18.

Suzuki M, Masuda Y . Effect of repeated electroconvulsive shock treatment on a depression model, mouse forced swimming. Tohoku J Exp Med. 2000; 189(1):83-6. DOI: 10.1620/tjem.189.83. View

19.

Gur E, Lerer B, Newman M . Chronic electroconvulsive shock and 5-HT autoreceptor activity in rat brain: an in vivo microdialysis study. J Neural Transm (Vienna). 1997; 104(8-9):795-804. DOI: 10.1007/BF01285548. View

20.

Codony X, Vela J, Javier Ramirez M . 5-HT(6) receptor and cognition. Curr Opin Pharmacol. 2011; 11(1):94-100. DOI: 10.1016/j.coph.2011.01.004. View