Clinical Nonlinear Laser Imaging of Human Skin: a Review

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Sep 25

PMID 25250337

Citations 13

Authors

Riccardo Cicchi

Dimitrios Kapsokalyvas

Francesco Saverio Pavone

Affiliations

Soon will be listed here.

Abstract

Nonlinear optical microscopy has the potential of being used in vivo as a noninvasive imaging modality for both epidermal and dermal imaging. This paper reviews the capabilities of nonlinear microscopy as a noninvasive high-resolution tool for clinical skin inspection. In particular, we show that two-photon fluorescence microscopy can be used as a diagnostic tool for characterizing epidermal layers by means of a morphological examination. Additional functional information on the metabolic state of cells can be provided by measuring the fluorescence decay of NADH. This approach allows differentiating epidermal layers having different structural and cytological features and has the potential of diagnosing pathologies in a very early stage. Regarding therapy follow-up, we demonstrate that nonlinear microscopy could be successfully used for monitoring the effect of a treatment. In particular, combined two-photon fluorescence and second-harmonic generation microscopy were used in vivo for monitoring collagen remodeling after microablative fractional laser resurfacing and for quantitatively monitoring psoriasis on the basis of the morphology of epidermal cells and dermal papillae. We believe that the described microscopic modalities could find in the near future a stable place in a clinical dermatological setting for quantitative diagnostic purposes and as a monitoring method for various treatments.

Citing Articles

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References

Campagnola P, Loew L . Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms. Nat Biotechnol. 2003; 21(11):1356-60. DOI: 10.1038/nbt894. View

Cicchi R, Sturiale A, Nesi G, Kapsokalyvas D, Alemanno G, Tonelli F . Multiphoton morpho-functional imaging of healthy colon mucosa, adenomatous polyp and adenocarcinoma. Biomed Opt Express. 2013; 4(7):1204-13. PMC: 3704099. DOI: 10.1364/BOE.4.001204. View

Fischer F, Volkmer B, Puschmann S, Greinert R, Breitbart W, Kiefer J . Risk estimation of skin damage due to ultrashort pulsed, focused near-infrared laser irradiation at 800 nm. J Biomed Opt. 2008; 13(4):041320. DOI: 10.1117/1.2960016. View

Cicchi R, Massi D, Sestini S, Carli P, de Giorgi V, Lotti T . Multidimensional non-linear laser imaging of Basal Cell Carcinoma. Opt Express. 2009; 15(16):10135-48. DOI: 10.1364/oe.15.010135. View

Gonzalez S, Gilaberte-Calzada Y . In vivo reflectance-mode confocal microscopy in clinical dermatology and cosmetology. Int J Cosmet Sci. 2008; 30(1):1-17. DOI: 10.1111/j.1468-2494.2008.00406.x. View

Moreaux L, Sandre O, Charpak S, Blanchard-Desce M, Mertz J . Coherent scattering in multi-harmonic light microscopy. Biophys J. 2001; 80(3):1568-74. PMC: 1301348. DOI: 10.1016/S0006-3495(01)76129-2. View

Dimitrow E, Riemann I, Ehlers A, Koehler M, Norgauer J, Elsner P . Spectral fluorescence lifetime detection and selective melanin imaging by multiphoton laser tomography for melanoma diagnosis. Exp Dermatol. 2009; 18(6):509-15. DOI: 10.1111/j.1600-0625.2008.00815.x. View

Masters B, So P . Confocal microscopy and multi-photon excitation microscopy of human skin in vivo. Opt Express. 2009; 8(1):2-10. DOI: 10.1364/oe.8.000002. View

Li D, Zheng W, Qu J . Time-resolved spectroscopic imaging reveals the fundamentals of cellular NADH fluorescence. Opt Lett. 2008; 33(20):2365-7. DOI: 10.1364/ol.33.002365. View

10.

Matteini P, Ratto F, Rossi F, Cicchi R, Stringari C, Kapsokalyvas D . Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging. Opt Express. 2009; 17(6):4868-78. DOI: 10.1364/oe.17.004868. View

11.

Brown E, McKee T, DiTomaso E, Pluen A, Seed B, Boucher Y . Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation. Nat Med. 2003; 9(6):796-800. DOI: 10.1038/nm879. View

12.

Konig K, Riemann I . High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. J Biomed Opt. 2003; 8(3):432-9. DOI: 10.1117/1.1577349. View

13.

Zipfel W, Williams R, Webb W . Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol. 2003; 21(11):1369-77. DOI: 10.1038/nbt899. View

14.

Huzaira M, Rius F, Rajadhyaksha M, Anderson R, Gonzalez S . Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy. J Invest Dermatol. 2001; 116(6):846-52. DOI: 10.1046/j.0022-202x.2001.01337.x. View

15.

Campagnola P, Millard A, Terasaki M, Hoppe P, Malone C, Mohler W . Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J. 2001; 82(1 Pt 1):493-508. PMC: 1302489. DOI: 10.1016/S0006-3495(02)75414-3. View

16.

Ulrich M, Lange-Asschenfeldt S, Gonzalez S . Clinical applicability of in vivo reflectance confocal microscopy in dermatology. G Ital Dermatol Venereol. 2012; 147(2):171-8. View

17.

Stoller P, Reiser K, Celliers P, Rubenchik A . Polarization-modulated second harmonic generation in collagen. Biophys J. 2002; 82(6):3330-42. PMC: 1302120. DOI: 10.1016/S0006-3495(02)75673-7. View

18.

Williams R, Zipfel W, Webb W . Interpreting second-harmonic generation images of collagen I fibrils. Biophys J. 2004; 88(2):1377-86. PMC: 1305140. DOI: 10.1529/biophysj.104.047308. View

19.

Scope A, Benvenuto-Andrade C, Agero A, Malvehy J, Puig S, Rajadhyaksha M . In vivo reflectance confocal microscopy imaging of melanocytic skin lesions: consensus terminology glossary and illustrative images. J Am Acad Dermatol. 2007; 57(4):644-58. DOI: 10.1016/j.jaad.2007.05.044. View

20.

Skala M, Riching K, Bird D, Gendron-Fitzpatrick A, Eickhoff J, Eliceiri K . In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia. J Biomed Opt. 2007; 12(2):024014. PMC: 2743958. DOI: 10.1117/1.2717503. View