The Protective Role of Vitamin E on the Testicular Tissue in Rats Exposed to Sodium Arsenite During the Prenatal Stage Till Sex Maturity: A Stereological Analysis

Overview

Journal Iran J Reprod Med

Specialty Reproductive Medicine

Date 2014 Sep 24

PMID 25246929

Citations 6

Authors

Malek Soleimani Mehranjani

Rezvan Taefi

Affiliations

Soon will be listed here.

Abstract

Background: Vitamin E is an effective antioxidant, protecting cells against oxidative stress.

Objective: In this investigation the protective effect of vitamin E on the testis during development and spermatogenesis in rats exposed to sodium arsenite was evaluated.

Materials And Methods: Pregnant Wistar rats were divided into 4 groups (n=8) control, sodium arsenite (8 mg/kg/day), sodium arsenite+vitamin E (100 mg/kg/day) and vitamin E. Treatment was carried out from day seven of pregnancy till 90 days. Finally the right testis was stereologically studied. The obtained data was analyzed using one way ANOVA and Tukey's test and the means difference was considered significant at p<0.05.

Results: The weight and volume of testis, volume of seminiferous tubules and its diameter, volume of interstitial tissue, height of germinal epithelium and the total number of types A and B spermatogonia, spermatocyte, spermatid and sertoli cells reduced significantly in sodium arsenite group compared to the control. Co-administration of vitamin E and sodium arsenite compensated the adverse effects of sodium arsenite on the above parameters.

Conclusion: We concluded co-treatment of rats with sodium arsenite and vitamin E could prevent the adverse effects of sodium arsenite exposure on the testicular tissue during the prenatal stage till sex maturity.

Citing Articles

Protective Effect of Gel against Cisplatin-Induced Testicular Damage, Sperm Alteration and Oxidative stress in Rats.

Erfani Majd N, Tabandeh M, Hosseinifar S, Sadeghi M Int J Fertil Steril. 2021; 15(3):210-218.

PMID: 34155868 PMC: 8233928. DOI: 10.22074/IJFS.2020.134691.

Transgenerational effects in DNA methylation, genotoxicity and reproductive phenotype by chronic arsenic exposure.

Nava-Rivera L, Betancourt-Martinez N, Lozoya-Martinez R, Carranza-Rosales P, Guzman-Delgado N, Carranza-Torres I Sci Rep. 2021; 11(1):8276.

PMID: 33859283 PMC: 8050275. DOI: 10.1038/s41598-021-87677-y.

Protective effects of vitamin E on cadmium-induced apoptosis in rat testes.

Amanpour P, Khodarahmi P, Salehipour M Naunyn Schmiedebergs Arch Pharmacol. 2019; 393(3):349-358.

PMID: 31620823 DOI: 10.1007/s00210-019-01736-w.

Protective potential of Vitamin E against methylphenidate-induced male gonadal changes in albino rats.

Iqbal S, Hameed U, Hasan B, Zia-Ul-Islam , Ahmed M, Brohi A Int J Health Sci (Qassim). 2019; 13(3):19-23.

PMID: 31123435 PMC: 6512147.

Effects of pentoxifylline administration on histomorphological parameters of streptozotocin-induced diabetic rat testes.

Piryaei A, Najar A, Bayat M Lab Anim Res. 2015; 31(3):111-6.

PMID: 26472963 PMC: 4602077. DOI: 10.5625/lar.2015.31.3.111.

References

El-Demerdash F . Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol. 2004; 18(1):113-21. DOI: 10.1016/j.jtemb.2004.04.001. View

Latendresse J, Warbrittion A, Jonassen H, Creasy D . Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicol Pathol. 2002; 30(4):524-33. DOI: 10.1080/01926230290105721. View

Sarkar M, Chaudhuri G, Chattopadhyay A, Biswas N . Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl. 2003; 5(1):27-31. View

Del Razo L, Styblo M, Cullen W, Thomas D . Determination of trivalent methylated arsenicals in biological matrices. Toxicol Appl Pharmacol. 2001; 174(3):282-93. DOI: 10.1006/taap.2001.9226. View

Chen H, Liu J, Luo L, Baig M, Kim J, Zirkin B . Vitamin E, aging and Leydig cell steroidogenesis. Exp Gerontol. 2005; 40(8-9):728-36. DOI: 10.1016/j.exger.2005.06.004. View

Waalkes M, Ward J, Liu J, Diwan B . Transplacental carcinogenicity of inorganic arsenic in the drinking water: induction of hepatic, ovarian, pulmonary, and adrenal tumors in mice. Toxicol Appl Pharmacol. 2003; 186(1):7-17. DOI: 10.1016/s0041-008x(02)00022-4. View

Sanghamitra S, Hazra J, Upadhyay S, Singh R, Amal R . Arsenic induced toxicity on testicular tissue of mice. Indian J Physiol Pharmacol. 2008; 52(1):84-90. View

Zhou D, Qiu S, Zhang J, Tian H, Wang H . The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J Androl. 2006; 8(5):584-8. DOI: 10.1111/j.1745-7262.2006.00198.x. View

Tseng C, Tseng C, Chiou H, Hsueh Y, Chong C, Chen C . Epidemiologic evidence of diabetogenic effect of arsenic. Toxicol Lett. 2002; 133(1):69-76. DOI: 10.1016/s0378-4274(02)00085-1. View

10.

Dalgaard M, Pilegaard K, Ladefoged O . In utero exposure to diethylstilboestrol or 4-n-nonylphenol in rats: number of sertoli cells, diameter and length of seminiferous tubules estimated by stereological methods. Pharmacol Toxicol. 2002; 90(2):59-65. DOI: 10.1034/j.1600-0773.2002.900202.x. View

11.

Chang S, Jin B, Youn P, Park C, Park J, Ryu D . Arsenic-induced toxicity and the protective role of ascorbic acid in mouse testis. Toxicol Appl Pharmacol. 2006; 218(2):196-203. DOI: 10.1016/j.taap.2006.11.009. View

12.

Pal S, Chatterjee A . Possible beneficial effects of melatonin supplementation on arsenic-induced oxidative stress in Wistar rats. Drug Chem Toxicol. 2006; 29(4):423-33. DOI: 10.1080/01480540600837993. View

13.

de Jager C, Bornman M, Van Der Horst G . The effect of p-nonylphenol, an environmental toxicant with oestrogenic properties, on fertility potential in adult male rats. Andrologia. 1999; 31(2):99-106. DOI: 10.1046/j.1439-0272.1999.00245.x. View

14.

Neiger R, Osweiler G . Effect of subacute low level dietary sodium arsenite on dogs. Fundam Appl Toxicol. 1989; 13(3):439-51. DOI: 10.1016/0272-0590(89)90281-9. View

15.

Hood R, Vedel G, Zaworotko M, Tatum F, Meeks R . Uptake, distribution, and metabolism of trivalent arsenic in the pregnant mouse. J Toxicol Environ Health. 1988; 25(4):423-34. DOI: 10.1080/15287398809531221. View

16.

Pant N, Shankar R, Srivastava S . In utero and lactational exposure of carbofuran to rats: effect on testes and sperm. Hum Exp Toxicol. 1997; 16(5):267-72. DOI: 10.1177/096032719701600506. View

17.

Mandarim-de-Lacerda C . Stereological tools in biomedical research. An Acad Bras Cienc. 2003; 75(4):469-86. DOI: 10.1590/s0001-37652003000400006. View

18.

Manna P, Sinha M, Sil P . Protection of arsenic-induced testicular oxidative stress by arjunolic acid. Redox Rep. 2008; 13(2):67-77. DOI: 10.1179/135100008X259169. View

19.

Chitra K, Mathur P . Vitamin E prevents nonylphenol-induced oxidative stress in testis of rats. Indian J Exp Biol. 2004; 42(2):220-3. View

20.

Chen A, Cao E, Zhang T, Qin J . Arsenite-induced reactive oxygen species and the repression of alpha-tocopherol in the MGC-803 cells. Eur J Pharmacol. 2002; 448(1):11-8. DOI: 10.1016/s0014-2999(02)01901-5. View