Phenotypic Characterisation of Saccharomyces Spp. for Tolerance to 1-butanol

Overview

Journal J Ind Microbiol Biotechnol

Publisher Oxford University Press

Specialty Biotechnology

Date 2014 Sep 23

PMID 25242291

Citations 2

Authors

A M Zaki

T T Wimalasena

D Greetham

Affiliations

Soon will be listed here.

Abstract

Biofuels are expected to play a role in replacing crude oil as a liquid transportation fuel, and research into butanol has highlighted the importance of this alcohol as a fuel. Butanol has a higher energy density than ethanol, butanol-gasoline blends do not separate in the presence of water, and butanol is miscible with gasoline (Szulczyk, Int J Energy Environ 1(1):2876-2895, 40). Saccharomyces cerevisiae has been used as a fermentative organism in the biofuel industry producing ethanol from glucose derived from starchy plant material; however, it typically cannot tolerate butanol concentrations greater than 2 % (Luong, Biotechnol Bioeng 29 (2):242-248, 27). 90 Saccharomyces spp. strains were screened for tolerance to 1-butanol via a phenotypic microarray assay and we observed significant variation in response with the most tolerant strains (S. cerevisiae DBVPG1788, S. cerevisiae DBVPG6044 and S. cerevisiae YPS128) exhibiting tolerance to 4 % 1-butanol compared with S. uvarum and S. castelli strains, which were sensitive to 3 % 1-butanol. Response to butanol was confirmed using traditional yeast methodologies such as growth; it was observed that fermentations in the presence of butanol, when using strains with a tolerant background, were significantly faster. Assessing for genetic rationale for tolerance, it was observed that 1-butanol-tolerant strains, when compared with 1-butanol-sensitive strains, had an up-regulation of RPN4, a transcription factor which regulates proteasome genes. Analysing for the importance of RPN4, we observed that a Δrpn4 strain displayed a reduced rate of fermentation in the presence of 1-butanol when compared with the BY4741 background strain. This data will aid the development of breeding programmes to produce better strains for future bio-butanol production.

Citing Articles

Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review.

Nawab S, Wang N, Ma X, Huo Y Microb Cell Fact. 2020; 19(1):79.

PMID: 32220254 PMC: 7099781. DOI: 10.1186/s12934-020-01337-w.

Rpn4 and proteasome-mediated yeast resistance to ethanol includes regulation of autophagy.

Bubis J, Spasskaya D, Gorshkov V, Kjeldsen F, Kofanova A, Lekanov D Appl Microbiol Biotechnol. 2020; 104(9):4027-4041.

PMID: 32157425 DOI: 10.1007/s00253-020-10518-x.

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