Rapid-scan EPR of Immobilized Nitroxides

Overview

Journal J Magn Reson

Publisher Elsevier

Date 2014 Sep 21

PMID 25240151

Citations 15

Authors

Zhelin Yu

Richard W Quine

George A Rinard

Mark Tseitlin

Hanan Elajaili

Velavan Kathirvelu

Laura J Clouston

Przemyslaw J Boratynski

Andrzej Rajca

Richard Stein

Hassane Mchaourab

Sandra S Eaton

Gareth R Eaton

Affiliations

Soon will be listed here.

Abstract

X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293 K. Scan widths were 155 G with 13.4 kHz scan frequency for (14)N-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100 G with 20.9 kHz scan frequency for (15)N-perdeuterated tempone. These wide scans were made possible by modifications to our rapid-scan driver, scan coils made of Litz wire, and the placement of highly conducting aluminum plates on the poles of a Bruker 10″ magnet to reduce resistive losses in the magnet pole faces. For the same data acquisition time, the signal-to-noise for the rapid-scan absorption spectra was about an order of magnitude higher than for continuous wave first-derivative spectra recorded with modulation amplitudes that do not broaden the lineshapes.

Citing Articles

Advances in rapid scan EPR spectroscopy.

Eaton G, Eaton S Methods Enzymol. 2022; 666:1-24.

PMID: 35465917 PMC: 10093151. DOI: 10.1016/bs.mie.2022.02.013.

Rapid Scan EPR imaging as a Tool for Magnetic Field Mapping.

Tseytlin O, Bobko A, Tseytlin M Appl Magn Reson. 2021; 51(9-10):1117-1124.

PMID: 33642700 PMC: 7909464. DOI: 10.1007/s00723-020-01238-6.

Spirocyclic Nitroxides as Versatile Tools in Modern Natural Sciences: From Synthesis to Applications. Part I. Old and New Synthetic Approaches to Spirocyclic Nitroxyl Radicals.

Zaytseva E, Mazhukin D Molecules. 2021; 26(3).

PMID: 33525514 PMC: 7865516. DOI: 10.3390/molecules26030677.

EPR Everywhere.

Biller J, McPeak J Appl Magn Reson. 2021; 52(8):1113-1139.

PMID: 33519097 PMC: 7826499. DOI: 10.1007/s00723-020-01304-z.

Full cycle rapid scan EPR deconvolution algorithm.

Tseytlin M J Magn Reson. 2017; 281:272-278.

PMID: 28666168 PMC: 5568913. DOI: 10.1016/j.jmr.2017.06.008.

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