Predicted 3D Structures of Olfactory Receptors with Details of Odorant Binding to OR1G1

Overview

Journal J Comput Aided Mol Des

Publisher Springer

Specialties Biomedical Engineering
Molecular Biology

Date 2014 Sep 17

PMID 25224127

Citations 9

Authors

Soo-Kyung Kim

William A Goddard 3rd

Affiliations

Soon will be listed here.

Abstract

Olfactory receptors (ORs) are responsible for mediating the sense of smell; they allow humans to recognize an enormous number of odors but the connection between binding and perception is not known. We predict the ensemble of low energy structures for the human OR1G1 (hOR1G1) and also for six other diverse ORs, using the G protein-coupled receptor Ensemble of Structures in Membrane BiLayer Environment complete sampling method that samples 13 trillion different rotations and tilts using four different templates to predict the 24 structures likely to be important in binding and activation. Our predicted most stable structures of hOR1G1 have a salt-bridge between the conserved D3.49 and K6.30 in the D(E)RY region, that we expect to be associated with an inactive form. The hOR1G1 structure also has specific interaction in transmembrane domains (TMD) 3-6 (E3.39 and H6.40), which is likely an important conformational feature for all hORs because of the ~94 to 98 % conservation among all hOR sequences. Of the five ligands studied (nonanal, 9-decen-1-ol, 1-nonanol, camphor, and n-butanal), we find that the 4 expected to bind lead to similar binding energies with nonanol the strongest.

Citing Articles

An overview on olfaction in the biological, analytical, computational, and machine learning fields.

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Combining the Classification and Pharmacophore Approaches to Understand Homogeneous Olfactory Perceptions at Peripheral Level: Focus on Two Aroma Mixtures.

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Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase.

de March C, Fukutani Y, Vihani A, Kida H, Matsunami H J Vis Exp. 2019; (146).

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