Lentivirus-mediated Knockdown of Krüppel-like Factor 9 Inhibits the Growth of Ovarian Cancer

Overview

Journal Arch Gynecol Obstet

Specialty Gynecology & Obstetrics

Date 2014 Sep 14

PMID 25216959

Citations 9

Authors

Qing-Hua Zhang

Hong-Tao Dou

Yu-Jie Tang

Shan Su

Pei-Shu Liu

Affiliations

Soon will be listed here.

Abstract

Purpose: Ovarian cancer is among the top diseases in the list of malignant gynaecologic tumors. In the present study, we aim to investigate the effect of lentivirus-mediated knockdown of Krüppel-like factor 9 (KLF9) on cell viability and tumor growth in ovarian cancer.

Methods: Firstly, the expression of KLF9 was determined by real-time PCR and western blot in human ovarian cancer tissues. Then, endogenous KLF9 expression was silenced by lentivirus in SKOV3 and OVCAR3 ovarian cancer cells, and followed by MTT and BrdU incorporation assays, cell cycle analysis and tumor xenografts in nude mice.

Results: Our results found that the expression of KLF9 is up-regulated in human ovarian cancer. As expected, KLF9 knockdown significantly inhibited cell proliferation and resulted in cell cycle arrest in the G0/G1 phase. Besides, KLF9 deficiency significantly inhibited tumor growth in nude mice.

Conclusion: Therefore, our data reveal that lentivirus-mediated KLF9 silencing might be promising in the treatment of human ovarian cancer.

Citing Articles

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Progesterone receptor inhibits the proliferation and invasion of endometrial cancer cells by up regulating Krüppel-like factor 9.

Yan X, Zhang H, Ke J, Zhang Y, Dai C, Zhu M Transl Cancer Res. 2022; 9(4):2220-2230.

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De Donato M, Babini G, Mozzetti S, Buttarelli M, Ciucci A, Arduini G J Exp Clin Cancer Res. 2020; 39(1):265.

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Jin Y, Yang L, Li X Exp Ther Med. 2020; 20(4):2953-2960.

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Pan F, Chen T, Sun X, Li K, Jiang X, Forsti A Front Oncol. 2019; 9:252.

PMID: 31024853 PMC: 6465763. DOI: 10.3389/fonc.2019.00252.