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Evaluation of Five Real-time PCR Assays for Detection of Mycoplasma Pneumoniae

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Specialty Microbiology
Date 2014 Sep 12
PMID 25210063
Citations 13
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Abstract

Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.

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