Prolonged Electrical Stimulation Causes No Damage to Sacral Nerve Roots in Rabbits

Overview

Journal Neural Regen Res

Date 2014 Sep 11

PMID 25206785

Citations 1

Authors

Peng Yan

Xiaohong Yang

Xiaoyu Yang

Weidong Zheng

Yunbing Tan

Affiliations

Soon will be listed here.

Abstract

Previous studies have shown that, anode block electrical stimulation of the sacral nerve root can produce physiological urination and reconstruct urinary bladder function in rabbits. However, whether long-term anode block electrical stimulation causes damage to the sacral nerve root remains unclear, and needs further investigation. In this study, a complete spinal cord injury model was established in New Zealand white rabbits through T9-10 segment transection. Rabbits were given continuous electrical stimulation for a short period and then chronic stimulation for a longer period. Results showed that compared with normal rabbits, the structure of nerve cells in the anterior sacral nerve roots was unchanged in spinal cord injury rabbits after electrical stimulation. There was no significant difference in the expression of apoptosis-related proteins such as Bax, Caspase-3, and Bcl-2. Experimental findings indicate that neurons in the rabbit sacral nerve roots tolerate electrical stimulation, even after long-term anode block electrical stimulation.

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References

Cook S, Sugden P, Clerk A . Regulation of bcl-2 family proteins during development and in response to oxidative stress in cardiac myocytes: association with changes in mitochondrial membrane potential. Circ Res. 1999; 85(10):940-9. DOI: 10.1161/01.res.85.10.940. View

Fehlings M, Tator C . The relationships among the severity of spinal cord injury, residual neurological function, axon counts, and counts of retrogradely labeled neurons after experimental spinal cord injury. Exp Neurol. 1995; 132(2):220-8. DOI: 10.1016/0014-4886(95)90027-6. View

Lewis J, Cheng E . Non-traditional management of the neurogenic bladder: tissue engineering and neuromodulation. ScientificWorldJournal. 2007; 7:1230-41. PMC: 5901112. DOI: 10.1100/tsw.2007.178. View

Jezernik S, Craggs M, Grill W, Creasey G, Rijkhoff N . Electrical stimulation for the treatment of bladder dysfunction: current status and future possibilities. Neurol Res. 2002; 24(5):413-30. DOI: 10.1179/016164102101200294. View

McCreery D, Pikov V, Lossinsky A, Bullara L, Agnew W . Arrays for chronic functional microstimulation of the lumbosacral spinal cord. IEEE Trans Neural Syst Rehabil Eng. 2004; 12(2):195-207. DOI: 10.1109/TNSRE.2004.827223. View

Nosseir M, Hinkel A, Pannek J . Clinical usefulness of urodynamic assessment for maintenance of bladder function in patients with spinal cord injury. Neurourol Urodyn. 2006; 26(2):228-33. DOI: 10.1002/nau.20319. View

Creasey G . Electrical stimulation of sacral roots for micturition after spinal cord injury. Urol Clin North Am. 1993; 20(3):505-15. View

Egon G, Barat M, Colombel P, Visentin C, Isambert J, Guerin J . Implantation of anterior sacral root stimulators combined with posterior sacral rhizotomy in spinal injury patients. World J Urol. 1998; 16(5):342-9. DOI: 10.1007/s003450050078. View

Hagerty J, Richards I, Kaplan W . Intravesical electrotherapy for neurogenic bladder dysfunction: a 22-year experience. J Urol. 2007; 178(4 Pt 2):1680-3. DOI: 10.1016/j.juro.2007.03.188. View

10.

Huber E, Kiss G, Berger T, Rehder P, Madersbacher H . [The value of intravesical electrostimulation in the treatment of acute prolonged bladder overdistension]. Urologe A. 2007; 46(6):662-6. DOI: 10.1007/s00120-007-1312-y. View

11.

Bamford J, Todd K, Mushahwar V . The effects of intraspinal microstimulation on spinal cord tissue in the rat. Biomaterials. 2010; 31(21):5552-63. PMC: 2875271. DOI: 10.1016/j.biomaterials.2010.03.051. View

12.

Ahn Y, Lee G, Kang S . Molecular insights of the injured lesions of rat spinal cords: Inflammation, apoptosis, and cell survival. Biochem Biophys Res Commun. 2006; 348(2):560-70. DOI: 10.1016/j.bbrc.2006.07.105. View

13.

Brindley G . Emptying the bladder by stimulating sacral ventral roots. J Physiol. 1974; 237(2):15P-16P. View

14.

Schalow G . Cure of urinary bladder functions in severe (95%) motoric complete cervical spinal cord injury in human. Electromyogr Clin Neurophysiol. 2010; 50(3-4):155-79. View

15.

Lee B, Haran M, Hunt L, Simpson J, Marial O, Rutkowski S . Spinal-injured neuropathic bladder antisepsis (SINBA) trial. Spinal Cord. 2006; 45(8):542-50. DOI: 10.1038/sj.sc.3101974. View

16.

Wang X, Gao Q, Yang X, Wang W, Gu X, Liu G . Long-term anodal block stimulation at sacral anterior roots promoted recovery of neurogenic bladder function in a rabbit model of complete spinal cord injury. Neural Regen Res. 2015; 7(5):352-8. PMC: 4350117. DOI: 10.3969/j.issn.1673-5374.2012.05.005. View