A Non-invasive, Rapid Method to Genotype Late-onset Alzheimer's Disease-related Apolipoprotein E Gene Polymorphisms

Overview

Journal Neural Regen Res

Date 2014 Sep 11

PMID 25206745

Citations 18

Authors

Li Yi

Ting Wu

Wenyuan Luo

Wen Zhou

Jun Wu

Affiliations

Soon will be listed here.

Abstract

The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer's disease cases.

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