Lateral Flow Test Using Echinococcus Granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2014 Sep 10

PMID 25200268

Citations 10

Authors

Akbar Khalilpour

Seyed Mahmoud Sadjjadi

Zohreh Kazemi Moghadam

Muhammad Hafiznur Yunus

Nor Dyana Zakaria

Sabariah Osman

Rahmah Noordin

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) caused by infection with Echinococcus granulosus is of major concern for humans in many parts of the world. Antigen B was prepared from E. granulosus hydatid fluid, and Western blots confirmed eight batches showing a band corresponding to the 8-/12-kDa subunit with positive serum and no low-molecular mass band (< 15 kDa) with negative serum. The batches were pooled and used to prepare lateral flow immunoglobulin G4 (IgG4) and IgG dipsticks. Diagnostic sensitivity was determined using serum samples from 21 hydatidosis patients, and diagnostic specificity was established using sera from 17 individuals infected with other parasites and 15 healthy people. IgG4 dipstick had a diagnostic sensitivity of 95% (20 of 21) and a specificity of 100% (32 of 32). The IgG dipstick had a sensitivity of 100% (21 of 21) and a specificity of 87.5% (28 of 32). Thus, both IgG and IgG4 dipsticks had high sensitivities, but IgG4 had greater specificity for the diagnosis of human CE.

Citing Articles

Comparison of Native Hydatid Cyst Fluid (HCF), Lyophilized HCF, Antigen B (AgB) and Lyophilized AgB (LAgB) Originated from Sensu Stricto for Sero-Diagnosis of Active, Transitional and Inactive Human Liver Cystic Echinococcosis.

Sadjjadi F, Mohammadzadeh T, Jafari S, Sharifi Y, Deilami H, Hafezi F Iran J Public Health. 2023; 52(8):1764-1772.

PMID: 37744545 PMC: 10512133. DOI: 10.18502/ijph.v52i8.13416.

Comparison of Isoenzyme Pattern of Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) Protoscoleces.

Dousti M, Sadjjadi S, Solgi R, Vafafar A, Sharifi Y, Radfar A Iran Biomed J. 2023; 27(2 & 3):136-45.

PMID: 37073115 PMC: 10314765. DOI: 10.61186/ibj.3815.

Evaluation of the Ability of Antigen B Originated from Sensu Stricto and for the Diagnosis of Confirmed Human Cystic Echinococcosis Using ELISA.

Sadjjadi S, Mohammadzadeh T, Hafezi F, Sadjjadi S Iran J Parasitol. 2022; 17(3):358-365.

PMID: 36466027 PMC: 9682382. DOI: 10.18502/ijpa.v17i3.10626.

Sero-Epidemiological Study of Human Hydatidosis in Semnan and Sorkheh, Semnan Province, Iran.

Hafezi F, Mohammadzadeh T, Pazoki R, Ranani K, Sadjjadi S Iran J Public Health. 2022; 51(6):1411-1418.

PMID: 36447961 PMC: 9659522. DOI: 10.18502/ijph.v51i6.9698.

Evaluation of the Diagnostic Performance of Recombinant Antigen B1 for Detection of Cystic Echinococcosis Using Lateral Flow Dipstick Test.

Noordin R, Khanbabaie S, Yunus M, Marti H, Nickel B, Harandi M Iran J Parasitol. 2020; 15(3):290-298.

PMID: 33082792 PMC: 7548468. DOI: 10.18502/ijpa.v15i3.4191.

References

Xu J, Feng T, Lin D, Wang Q, Tang L, Wu X . Performance of a dipstick dye immunoassay for rapid screening of Schistosoma japonicum infection in areas of low endemicity. Parasit Vectors. 2011; 4:87. PMC: 3123290. DOI: 10.1186/1756-3305-4-87. View

Ortona E, Rigano R, Buttari B, Delunardo F, Ioppolo S, Margutti P . An update on immunodiagnosis of cystic echinococcosis. Acta Trop. 2003; 85(2):165-71. DOI: 10.1016/s0001-706x(02)00225-5. View

van Doorn H, Koelewijn R, Hofwegen H, Gilis H, Wetsteyn J, Wismans P . Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans. J Clin Microbiol. 2006; 45(2):438-42. PMC: 1829047. DOI: 10.1128/JCM.01735-06. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Saidin S, Yunus M, Zakaria N, Razak K, Huat L, Othman N . Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis. 2014; 14:182. PMC: 3986461. DOI: 10.1186/1471-2334-14-182. View

Ito A . Serologic and molecular diagnosis of zoonotic larval cestode infections. Parasitol Int. 2002; 51(3):221-35. DOI: 10.1016/s1383-5769(02)00036-3. View

Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y . Recent advances in characterization of Echinococcus antigen B. Parasitol Int. 2005; 55 Suppl:S57-62. DOI: 10.1016/j.parint.2005.11.008. View

Mohammadzadeh T, Sako Y, Sadjjadi S, Sarkari B, Ito A . Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-5. DOI: 10.1016/j.trstmh.2012.01.012. View

Feng X, Wen H, Zhang Z, Chen X, Ma X, Zhang J . Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis. Acta Trop. 2009; 113(2):114-20. DOI: 10.1016/j.actatropica.2009.10.003. View

10.

Sbihi Y, Gil J, Alvarez P, Orduna A, Rodriguez-Torres A, Osuna A . Development of a dipstick dye immunoassay for diagnosing hydatidosis. J Clin Lab Anal. 2003; 17(6):219-22. PMC: 6808177. DOI: 10.1002/jcla.10097. View

11.

Gomara M, Haro I . Synthetic peptides for the immunodiagnosis of human diseases. Curr Med Chem. 2007; 14(5):531-46. DOI: 10.2174/092986707780059698. View

12.

Lorenzo C, Last J, Gonzalez-Sapienza G . The immunogenicity of Echinococcus granulosus antigen 5 is determined by its post-translational modifications. Parasitology. 2005; 131(Pt 5):669-77. DOI: 10.1017/S0031182005008309. View

13.

MADDISON S, Slemenda S, Schantz P, Fried J, Wilson M, Tsang V . A specific diagnostic antigen of Echinococcus granulosus with an apparent molecular weight of 8 kDA. Am J Trop Med Hyg. 1989; 40(4):377-83. DOI: 10.4269/ajtmh.1989.40.377. View

14.

Hewitson J, Grainger J, Maizels R . Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity. Mol Biochem Parasitol. 2009; 167(1):1-11. PMC: 2706953. DOI: 10.1016/j.molbiopara.2009.04.008. View

15.

Sadjjadi S, Sedaghat F, Hosseini S, Sarkari B . Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-7. PMC: 2688797. DOI: 10.3347/kjp.2009.47.2.153. View

16.

Rahmah N, Shenoy R, Nutman T, Weiss N, Gilmour K, Maizels R . Multicentre laboratory evaluation of Brugia Rapid dipstick test for detection of brugian filariasis. Trop Med Int Health. 2003; 8(10):895-900. DOI: 10.1046/j.1365-3156.2003.01102.x. View

17.

Carmena D, Benito A, Eraso E . Antigens for the immunodiagnosis of Echinococcus granulosus infection: An update. Acta Trop. 2006; 98(1):74-86. DOI: 10.1016/j.actatropica.2006.02.002. View

18.

Craig P, Rogan M, Campos-Ponce M . Echinococcosis: disease, detection and transmission. Parasitology. 2004; 127 Suppl:S5-20. View

19.

Zhang W, Li J, McManus D . Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev. 2003; 16(1):18-36. PMC: 145297. DOI: 10.1128/CMR.16.1.18-36.2003. View

20.

Makhsin S, Razak K, Noordin R, Zakaria N, Chun T . The effects of size and synthesis methods of gold nanoparticle-conjugated MαHIgG4 for use in an immunochromatographic strip test to detect brugian filariasis. Nanotechnology. 2012; 23(49):495719. DOI: 10.1088/0957-4484/23/49/495719. View