Validation of Analytical Breast Cancer Microarray Analysis in Medical Laboratory

Overview

Journal Med Oncol

Publisher Springer

Specialty Oncology

Date 2014 Sep 4

PMID 25182704

Citations 2

Authors

Amal Said Darweesh

Manal Louis Louka

Maha Hana

Shaymaa Rashad

Mohamed El-Shinawi

Ahmed Sharaf-Eldin

Samar Kamal Kassim

Affiliations

Soon will be listed here.

Abstract

A previously reported microarray data analysis by RISS algorithm on breast cancer showed over-expression of the growth factor receptor (Grb7) and it also highlighted Tweety (TTYH1) gene to be under expressed in breast cancer for the first time. Our aim was to validate the results obtained from the microarray analysis with respect to these genes. Also, the relationship between their expression and the different prognostic indicators was addressed. RNA was extracted from the breast tissue of 30 patients with primary malignant breast cancer. Control samples from the same patients were harvested at a distance of ≥5 cm from the tumour. Semi-quantitative RT-PCR analysis was done on all samples. There was a significant difference between the malignant and control tissues as regards Grb7 expression. It was significantly related to the presence of lymph node metastasis, stage and histological grade of the malignant tumours. There was a significant inverse relation between expression of Grb7 and expression of both oestrogen and progesterone receptors. Grb7 was found to be significantly related to the biological classification of breast cancer. TTYH1 was not expressed in either the malignant or the control samples. The RISS by our group algorithm developed was laboratory validated for Grb7, but not for TTYH1. The newly developed software tool needs to be improved.

Citing Articles

The Gene Family: From Embryo to Disease.

Nalamalapu R, Yue M, Stone A, Murphy S, Saha M Front Mol Neurosci. 2021; 14:672511.

PMID: 34262434 PMC: 8273234. DOI: 10.3389/fnmol.2021.672511.

Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer.

Zeng M, Yang Z, Hu X, Liu Y, Yang X, Ran H Int J Clin Exp Pathol. 2015; 8(9):11296-304.

PMID: 26617853 PMC: 4637669.

References

Giricz O, Calvo V, Pero S, Krag D, Sparano J, Kenny P . GRB7 is required for triple-negative breast cancer cell invasion and survival. Breast Cancer Res Treat. 2011; 133(2):607-15. DOI: 10.1007/s10549-011-1822-6. View

Pradip D, Bouzyk M, Dey N, Leyland-Jones B . Dissecting GRB7-mediated signals for proliferation and migration in HER2 overexpressing breast tumor cells: GTP-ase rules. Am J Cancer Res. 2013; 3(2):173-95. PMC: 3623837. View

Chu P, Li T, Ding S, Lai I, Shen T . EGF-induced Grb7 recruits and promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells. J Biol Chem. 2010; 285(38):29279-85. PMC: 2937960. DOI: 10.1074/jbc.C110.114124. View

Sen S, Sharma S, Gupta A, Gupta N, Singh H, Roychoudhury A . Molecular characterization of explant cultured human oral mucosal epithelial cells. Invest Ophthalmol Vis Sci. 2011; 52(13):9548-54. DOI: 10.1167/iovs.11-7946. View

Ramsey B, Bai T, Newell A, Troxell M, Park B, Olson S . GRB7 protein over-expression and clinical outcome in breast cancer. Breast Cancer Res Treat. 2010; 127(3):659-69. DOI: 10.1007/s10549-010-1010-0. View

Sahlberg K, Hongisto V, Edgren H, Makela R, Hellstrom K, Due E . The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells. Mol Oncol. 2012; 7(3):392-401. PMC: 5528495. DOI: 10.1016/j.molonc.2012.10.012. View

Altieri D . Validating survivin as a cancer therapeutic target. Nat Rev Cancer. 2003; 3(1):46-54. DOI: 10.1038/nrc968. View

Campbell H, Kamei M, Claudianos C, Woollatt E, Sutherland G, Suzuki Y . Human and mouse homologues of the Drosophila melanogaster tweety (tty) gene: a novel gene family encoding predicted transmembrane proteins. Genomics. 2000; 68(1):89-92. DOI: 10.1006/geno.2000.6259. View

Parisi F, Gonzalez A, Nadler Y, Camp R, Rimm D, Kluger H . Benefits of biomarker selection and clinico-pathological covariate inclusion in breast cancer prognostic models. Breast Cancer Res. 2010; 12(5):R66. PMC: 3096952. DOI: 10.1186/bcr2633. View

10.

Carey L, Perou C, Livasy C, Dressler L, Cowan D, Conway K . Race, breast cancer subtypes, and survival in the Carolina Breast Cancer Study. JAMA. 2006; 295(21):2492-502. DOI: 10.1001/jama.295.21.2492. View

11.

Bai T, Luoh S . GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation. Carcinogenesis. 2007; 29(3):473-9. DOI: 10.1093/carcin/bgm221. View

12.

Nadler Y, Gonzalez A, Camp R, Rimm D, Kluger H, Kluger Y . Growth factor receptor-bound protein-7 (Grb7) as a prognostic marker and therapeutic target in breast cancer. Ann Oncol. 2009; 21(3):466-473. PMC: 2826097. DOI: 10.1093/annonc/mdp346. View

13.

Salem A, Salem M, Abbass H . Breast cancer: surgery at the South egypt cancer institute. Cancers (Basel). 2013; 2(4):1771-8. PMC: 3840361. DOI: 10.3390/cancers2041771. View

14.

Toiyama Y, Mizoguchi A, Kimura K, Hiro J, Inoue Y, Tutumi T . TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation. World J Gastroenterol. 2007; 13(19):2717-21. PMC: 4147121. DOI: 10.3748/wjg.v13.i19.2717. View

15.

Cariou B, Bereziat V, Moncoq K, Kasus-Jacobi A, Perdereau D, Le Marcis V . Regulation and functional roles of Grb14. Front Biosci. 2004; 9:1626-36. DOI: 10.2741/1228. View

16.

Vinatzer U, Dampier B, Streubel B, Pacher M, Seewald M, Stratowa C . Expression of HER2 and the coamplified genes GRB7 and MLN64 in human breast cancer: quantitative real-time reverse transcription-PCR as a diagnostic alternative to immunohistochemistry and fluorescence in situ hybridization. Clin Cancer Res. 2005; 11(23):8348-57. DOI: 10.1158/1078-0432.CCR-05-0841. View