Efficacy of Essential Oils of Thymus Vulgaris and Origanum Vulgare on Echinococcus Granulosus

Overview

Journal Interdiscip Perspect Infect Dis

Publisher Wiley

Date 2014 Sep 3

PMID 25180033

Citations 13

Authors

P E Pensel

M A Maggiore

L B Gende

M J Eguaras

M G Denegri

M C Elissondo

Affiliations

Soon will be listed here.

Abstract

The aim of the present work was to determine the in vitro effect of T. vulgaris and O. vulgare essential oils against E. granulosus protoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated with O. vulgare after 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated with T. vulgaris treated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol. T. vulgaris and O. vulgare essential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy of T. vulgaris and O. vulgare essential oils was also demonstrated in vitro on E. granulosus murine cysts. Our data suggest that essential oils of T. vulgaris and O. vulgare have anthelmintic effect against protoscoleces and cysts of E. granulosus.

Citing Articles

In vitro and ex vivo protoscolicidal effect of poly(amidoamine) nanoemulsion against Echinococcus granulosus.

Aboelsoued D, Toaleb N, Ibrahim S, Ibrahim S Sci Rep. 2024; 14(1):11957.

PMID: 38796499 PMC: 11127951. DOI: 10.1038/s41598-024-62015-0.

Evaluation of structural changes of protoscoleces following exposure to different protoscolicidal solutions evaluated by differential interference contrast microscopy.

Karpisheh E, Sadjjadi S, Nekooeian A, Sharifi Y J Parasit Dis. 2023; 47(4):850-858.

PMID: 38009156 PMC: 10667190. DOI: 10.1007/s12639-023-01632-4.

Antiparasitic treatment using herbs and spices: A review of the literature of the phytotherapy.

Strothmann A, Berne M, Capella G, Moura M, da Silva Terto W, da Costa C Braz J Vet Med. 2022; 44:e004722.

PMID: 36381138 PMC: 9652050. DOI: 10.29374/2527-2179.bjvm004722.

Antiparasitic Effects of Asteraceae Species Extracts on s.s.

Albani C, Borgo J, Fabbri J, Pensel P, Paladini A, Beer M Evid Based Complement Alternat Med. 2022; 2022:6371849.

PMID: 36193140 PMC: 9526667. DOI: 10.1155/2022/6371849.

Herbal Medicines against Hydatid Disease: A Systematic Review (2000-2021).

Alvi M, Khan S, Ali R, Qamar W, Saqib M, Faridi N Life (Basel). 2022; 12(5).

PMID: 35629345 PMC: 9145516. DOI: 10.3390/life12050676.

References

Flohr C, Tuyen L, Lewis S, Minh T, Campbell J, Britton J . Low efficacy of mebendazole against hookworm in Vietnam: two randomized controlled trials. Am J Trop Med Hyg. 2007; 76(4):732-6. View

James C, Hudson A, Davey M . Drug resistance mechanisms in helminths: is it survival of the fittest?. Trends Parasitol. 2009; 25(7):328-35. DOI: 10.1016/j.pt.2009.04.004. View

Maggiore M, Albanese A, Gende L, Eguaras M, Denegri G, Elissondo M . Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes. Parasitol Res. 2011; 110(3):1103-12. DOI: 10.1007/s00436-011-2595-x. View

Cumino A, Nicolao M, Loos J, Denegri G, Elissondo M . Echinococcus granulosus tegumental enzymes as in vitro markers of pharmacological damage: a biochemical and molecular approach. Parasitol Int. 2012; 61(4):579-85. DOI: 10.1016/j.parint.2012.05.007. View

Elissondo M, Pensel P, Denegri G . Could thymol have effectiveness on scolices and germinal layer of hydatid cysts?. Acta Trop. 2012; 125(3):251-7. DOI: 10.1016/j.actatropica.2012.12.007. View

Szasz G . A kinetic photometric method for serum gamma-glutamyl transpeptidase. Clin Chem. 1969; 15(2):124-36. View

Brunetti E, Kern P, Vuitton D . Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop. 2009; 114(1):1-16. DOI: 10.1016/j.actatropica.2009.11.001. View

Camurca-Vasconcelos A, Bevilaqua C, Morais S, Maciel M, Costa C, Macedo I . Anthelmintic activity of Croton zehntneri and Lippia sidoides essential oils. Vet Parasitol. 2007; 148(3-4):288-94. DOI: 10.1016/j.vetpar.2007.06.012. View

Budke C, Deplazes P, Torgerson P . Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis. 2006; 12(2):296-303. PMC: 3373106. DOI: 10.3201/eid1202.050499. View

10.

Masotti V, Juteau F, Bessiere J, Viano J . Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. J Agric Food Chem. 2003; 51(24):7115-21. DOI: 10.1021/jf034621y. View

11.

Bakkali F, Averbeck S, Averbeck D, Idaomar M . Biological effects of essential oils--a review. Food Chem Toxicol. 2007; 46(2):446-75. DOI: 10.1016/j.fct.2007.09.106. View

12.

Elissondo M, Ceballos L, Alvarez L, Sanchez Bruni S, Lanusse C, Denegri G . Flubendazole and ivermectin in vitro combination therapy produces a marked effect on Echinococcus granulosus protoscoleces and metacestodes. Parasitol Res. 2009; 105(3):835-42. DOI: 10.1007/s00436-009-1469-y. View

13.

Skandamis P, Nychas G . Effect of oregano essential oil on microbiological and physico-chemical attributes of minced meat stored in air and modified atmospheres. J Appl Microbiol. 2002; 91(6):1011-22. DOI: 10.1046/j.1365-2672.2001.01467.x. View

14.

Armstrong J . Mitochondrial membrane permeabilization: the sine qua non for cell death. Bioessays. 2006; 28(3):253-60. DOI: 10.1002/bies.20370. View

15.

Edris A . Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res. 2007; 21(4):308-23. DOI: 10.1002/ptr.2072. View

16.

Githiori J, Athanasiadou S, Thamsborg S . Use of plants in novel approaches for control of gastrointestinal helminths in livestock with emphasis on small ruminants. Vet Parasitol. 2006; 139(4):308-20. DOI: 10.1016/j.vetpar.2006.04.021. View

17.

De S, Pan D, Bera A, Sreevatsava V, Bandyopadhyay S, Chaudhuri D . In vitro assessment of praziquantel and a novel nanomaterial against protoscoleces of Echinococcus granulosus. J Helminthol. 2011; 86(1):26-9. DOI: 10.1017/S0022149X10000908. View

18.

Spicher M, Naguleswaran A, Ortega-Mora L, Muller J, Gottstein B, Hemphill A . In vitro and in vivo effects of 2-methoxyestradiol, either alone or combined with albendazole, against Echinococcus metacestodes. Exp Parasitol. 2008; 119(4):475-482. DOI: 10.1016/j.exppara.2008.02.012. View

19.

Chang H, Hsu S, Chou C, Cheng J, Wang J, Lin K . Effect of thymol on Ca2+ homeostasis and viability in MG63 human osteosarcoma cells. Pharmacology. 2011; 88(3-4):201-12. DOI: 10.1159/000331864. View

20.

Arana-Sanchez A, Estarron-Espinosa M, Obledo-Vazquez E, Padilla-Camberos E, Silva-Vazquez R, Lugo-Cervantes E . Antimicrobial and antioxidant activities of Mexican oregano essential oils (Lippia graveolens H. B. K.) with different composition when microencapsulated in beta-cyclodextrin. Lett Appl Microbiol. 2010; 50(6):585-90. DOI: 10.1111/j.1472-765X.2010.02837.x. View