Analysis of Papaya Cell Wall-related Genes During Fruit Ripening Indicates a Central Role of Polygalacturonases During Pulp Softening

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Aug 28

PMID 25162506

Citations 32

Authors

Joao Paulo Fabi

Sabrina Garcia Broetto

Sarah Ligia Garcia Leme da Silva

Silin Zhong

Franco Maria Lajolo

Joao Roberto Oliveira do Nascimento

Affiliations

Soon will be listed here.

Abstract

Papaya (Carica papaya L.) is a climacteric fleshy fruit that undergoes dramatic changes during ripening, most noticeably a severe pulp softening. However, little is known regarding the genetics of the cell wall metabolism in papayas. The present work describes the identification and characterization of genes related to pulp softening. We used gene expression profiling to analyze the correlations and co-expression networks of cell wall-related genes, and the results suggest that papaya pulp softening is accomplished by the interactions of multiple glycoside hydrolases. The polygalacturonase cpPG1 appeared to play a central role in the network and was further studied. The transient expression of cpPG1 in papaya results in pulp softening and leaf necrosis in the absence of ethylene action and confirms its role in papaya fruit ripening.

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