Nuclear Export of the Influenza Virus Ribonucleoprotein Complex: Interaction of Hsc70 with Viral Proteins M1 and NS2

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2014 Aug 28

PMID 25161876

Citations 11

Authors

Ken Watanabe

Teppei Shimizu

Saiko Noda

Fujiko Tsukahara

Yoshiro Maru

Nobuyuki Kobayashi

Affiliations

Soon will be listed here.

Abstract

The influenza virus replicates in the host cell nucleus, and the progeny viral ribonucleoprotein complex (vRNP) is exported to the cytoplasm prior to maturation. NS2 has a nuclear export signal that mediates the nuclear export of vRNP by the vRNP-M1-NS2 complex. We previously reported that the heat shock cognate 70 (Hsc70) protein binds to M1 protein and mediates vRNP export. However, the interactions among M1, NS2, and Hsc70 are poorly understood. In the present study, we demonstrate that Hsc70 interacts with M1 more strongly than with NS2 and competes with NS2 for M1 binding, suggesting an important role of Hsc70 in the nuclear export of vRNP.

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