Reversal Effect of Vitamin D on Different Multidrug-resistant Cells
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We investigated the reversal effect of vitamin D on the multidrug-resistant leukemic Jurkat/ADR and K562/ADR cell lines and conducted a preliminary investigation of its reversal mechanism. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the reversal effect of vitamin D on multidrug-resistant cells. Real-time polymerase chain reaction was used to determine the effect of vitamin D on intracellular expression of mRNA of the multidrug-resistant gene (MDRI) and the multidrug-resistance-related gene (MRP1). A protein quantitative analysis method was used to determine the effect of vitamin D on intracellular glutathione content. After treatment of Jurkat/ADR and K562/ADR cells with vitamin D, multidrug resistance was reversed in a dose-dependent manner, which may have reduced mRNA expression of the MDR1 and MRP1 genes, the P-glycoprotein content on the cell surface, and the intracellular glutathione level. Different concentrations of vitamin D showed varying reversal effects on different multidrug-resistant cells. The resistance mechanism may be related to the inhibition of the expression of MDR1 and MRP1 genes.
Peixoto D, Ravasco J, Blanco-Fernandez B, Veiga F, Concheiro A, Conde J Mater Today Bio. 2025; 31:101555.
PMID: 40026626 PMC: 11869029. DOI: 10.1016/j.mtbio.2025.101555.
Digvijaya , Mittal S, Mittal P, Singh R, Gupta S, Singh T Scientifica (Cairo). 2024; 2024:9978076.
PMID: 39618688 PMC: 11606658. DOI: 10.1155/2024/9978076.
Negri M, Gentile A, de Angelis C, Monto T, Patalano R, Colao A Nutrients. 2020; 12(6).
PMID: 32560347 PMC: 7353389. DOI: 10.3390/nu12061798.
Tan K, Sampson A, Osa-Andrews B, Iram S Drug Metab Dispos. 2018; 46(12):1856-1866.
PMID: 30232176 PMC: 7333660. DOI: 10.1124/dmd.118.081612.
Maayah Z, Zhang T, Forrest M, Alrushaid S, Doschak M, Davies N Pharmaceutics. 2018; 10(3).
PMID: 30181466 PMC: 6161239. DOI: 10.3390/pharmaceutics10030144.