The Iho670 Fibers of Ignicoccus Hospitalis Are Anchored in the Cell by a Spherical Structure Located Beneath the Inner Membrane

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2014 Aug 27

PMID 25157085

Citations 5

Authors

Carolin Meyer

Thomas Heimerl

Reinhard Wirth

Andreas Klingl

Reinhard Rachel

Affiliations

Soon will be listed here.

Abstract

The Iho670 fibers of the hyperthermophilic crenarchaeon of Ignicoccus hospitalis were shown to contain several features that indicate them as type IV pilus-like structures. The application of different visualization methods, including electron tomography and the reconstruction of a three-dimensional model, enabled a detailed description of a hitherto undescribed anchoring structure of the cell appendages. It could be identified as a spherical structure beneath the inner membrane. Furthermore, pools of the fiber protein Iho670 could be localized in the inner as well as the outer cellular membrane of I. hospitalis cells and in the tubes/vesicles in the intermembrane compartment by immunological methods.

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