The Environmental Pollutant Cadmium Induces Homeostasis Alteration in Muscle Cells in Vitro

Overview

Journal J Endocrinol Invest

Publisher Springer

Specialty Endocrinology

Date 2014 Aug 24

PMID 25149082

Citations 5

Authors

V Papa

F Wannenes

C Crescioli

D Caporossi

A Lenzi

S Migliaccio

L Di Luigi

Affiliations

Soon will be listed here.

Abstract

Background: Cadmium (Cd) is a heavy metal widely distributed throughout the environment as a result of contamination from a variety of sources. It exerts toxic effects in many tissues but scarce data are present as yet on potential effects on skeletal muscle tissue.

Aim: To evaluate the potential alteration induced by Cd in skeletal muscle cells.

Materials And Methods: C2C12 skeletal muscle cells were treated with Cd at different times of cellular differentiation and gene expression was evaluated.

Results: Exposure to Cd decreased significantly p21 mRNA expression and strongly up-regulated cyclin D1 mRNA expression in committed cells and in differentiated myotubes. Moreover, myogenin, fast MyHC-IIb and slow MyHC-I mRNAs expression were also significantly decreased both in committed cells and in myotubes. Moreover, Cd exposure induced a strong increase of Pax3, Pax7 and Myf5 mRNAs expression and stimulated an up-regulation of IL6 and TNF-α proinflammatory cytokines.

Conclusion: These data lead to hypothesize that environmental Cd exposure might trigger an injury-like event in muscle tissue, possibly by an estrogen receptor-mediated mechanism.

Citing Articles

Hexavalent chromium inhibits myogenic differentiation and induces myotube atrophy.

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Cadmium in Human Diseases: It's More than Just a Mere Metal.

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Cadmium exposure alters steroid receptors and proinflammatory cytokine levels in endothelial cells in vitro: a potential mechanism of endocrine disruptor atherogenic effect.

Fittipaldi S, Bimonte V, Soricelli A, Aversa A, Lenzi A, Greco E J Endocrinol Invest. 2018; 42(6):727-739.

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Structural and functional changes in the zebrafish (Danio rerio) skeletal muscle after cadmium exposure.

Avallone B, Agnisola C, Cerciello R, Panzuto R, Simoniello P, Creti P Cell Biol Toxicol. 2015; 31(6):273-83.

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The endocrine disruptor cadmium alters human osteoblast-like Saos-2 cells homeostasis in vitro by alteration of Wnt/β-catenin pathway and activation of caspases.

Papa V, Bimonte V, Wannenes F, DAbusco A, Fittipaldi S, Scandurra R J Endocrinol Invest. 2015; 38(12):1345-56.

PMID: 26335301 DOI: 10.1007/s40618-015-0380-x.

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