Investigation of Class I Integron in Salmonella Infantis and Its Association With Drug Resistance

Overview

Journal Jundishapur J Microbiol

Publisher Brieflands

Specialty Microbiology

Date 2014 Aug 23

PMID 25147710

Citations 8

Authors

Fariba Asgharpour

Ramazan Rajabnia

Elaheh Ferdosi Shahandashti

Mahmood Amin Marashi

Mahya Khalilian

Zahra Moulana

Affiliations

Soon will be listed here.

Abstract

Background: Infection with non-typhoid Salmonella (NTS) is one of the most important health problems all over the world. Antimicrobial drug resistance is increasing among Salmonella infantis species.

Objectives: The aim of this study was to investigate the frequency of presence of class 1 integrons in S. infantis species as well as its association with drug resistance.

Materials And Methods: This cross-sectional study was performed on 50 S. infantis isolated strains, collected from chicken samples between 2009-2011. These strains were identified by standard biochemical tests and serology. Antibiotic susceptibility profiles and minimum inhibitory concentration determination for 14 antibacterial agents were performed using micro dilution and disk diffusion methods. The detection of class 1 integron was performed by the PCR method. The demographic and microbiological data for the integron positive and negative isolates were compared by SPSS software.

Results: Eighteen out of 50 (36%) of isolated S. infantis species had intl gene. The isolated bacteria were sensitive to cefotaxime and ciprofloxacin (100%). Also isolates were resistant to nalidixic acid, tetracycline and streptomycin. All isolate with class 1 integron were multidrug resistant.

Conclusions: The result of this study showed that due to increased level of drug resistance in S. infantis and the presence of class 1 integron in these strains, resistance can be transferred to other food borne pathogens.

Citing Articles

Integron and its role in antimicrobial resistance: A literature review on some bacterial pathogens.

Sabbagh P, Rajabnia M, Maali A, Ferdosi-Shahandashti E Iran J Basic Med Sci. 2021; 24(2):136-142.

PMID: 33953851 PMC: 8061329. DOI: 10.22038/ijbms.2020.48905.11208.

Class I integrons among multidrug resistant isolates from hospitalized patients in Babol, North of Iran.

Ghasemi E, Ferdosi-Shahandashti E, Rajabnia M, Sabbagh P, Maali A, Ferdosi-Shahandashti A Caspian J Intern Med. 2021; 12(1):65-69.

PMID: 33680400 PMC: 7919182. DOI: 10.22088/cjim.12.1.65.

Antibiotic resistance profiles of serotypes isolated from animals in Iran: a meta-analysis.

Vaez H, Ghanbari F, Sahebkar A, Khademi F Iran J Vet Res. 2020; 21(3):188-197.

PMID: 33178296 PMC: 7608045.

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Infantis Strains Isolated from Chicken Meat: First Findings in Chile.

Lapierre L, Cornejo J, Zavala S, Galarce N, Sanchez F, Benavides M Animals (Basel). 2020; 10(6).

PMID: 32570768 PMC: 7341295. DOI: 10.3390/ani10061049.

Resistance integrons; A Mini review.

Akrami F, Rajabnia M, Pournajaf A Caspian J Intern Med. 2019; 10(4):370-376.

PMID: 31814933 PMC: 6856922. DOI: 10.22088/cjim.10.4.370.

References

Ashtiani M, Monajemzadeh M, Kashi L . Trends in antimicrobial resistance of fecal Shigella and Salmonella isolates in Tehran, Iran. Indian J Pathol Microbiol. 2009; 52(1):52-5. DOI: 10.4103/0377-4929.44964. View

Antunes P, Machado J, Sousa J, Peixe L . Dissemination amongst humans and food products of animal origin of a Salmonella typhimurium clone expressing an integron-borne OXA-30 beta-lactamase. J Antimicrob Chemother. 2004; 54(2):429-34. DOI: 10.1093/jac/dkh333. View

Fabrega A, Sanchez-Cespedes J, Soto S, Vila J . Quinolone resistance in the food chain. Int J Antimicrob Agents. 2008; 31(4):307-15. DOI: 10.1016/j.ijantimicag.2007.12.010. View

Parry C, Threlfall E . Antimicrobial resistance in typhoidal and nontyphoidal salmonellae. Curr Opin Infect Dis. 2008; 21(5):531-8. DOI: 10.1097/QCO.0b013e32830f453a. View

Yan H, Li L, Alam M, Shinoda S, Miyoshi S, Shi L . Prevalence and antimicrobial resistance of Salmonella in retail foods in northern China. Int J Food Microbiol. 2010; 143(3):230-4. DOI: 10.1016/j.ijfoodmicro.2010.07.034. View

Shahada F, Chuma T, Tobata T, Okamoto K, Sueyoshi M, Takase K . Molecular epidemiology of antimicrobial resistance among Salmonella enterica serovar Infantis from poultry in Kagoshima, Japan. Int J Antimicrob Agents. 2006; 28(4):302-7. DOI: 10.1016/j.ijantimicag.2006.07.003. View

Dahshan H, Chuma T, Shahada F, Akiba M, Fujimoto H, Akasaka K . Characterization of antibiotic resistance and the emergence of AmpC-producing Salmonella Infantis from pigs. J Vet Med Sci. 2010; 72(11):1437-42. DOI: 10.1292/jvms.10-0186. View

Rahmani M, Peighambari S, Svendsen C, Cavaco L, Agerso Y, Hendriksen R . Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Vet Res. 2013; 9:66. PMC: 3623788. DOI: 10.1186/1746-6148-9-66. View

Wannaprasat W, Padungtod P, Chuanchuen R . Class 1 integrons and virulence genes in Salmonella enterica isolates from pork and humans. Int J Antimicrob Agents. 2011; 37(5):457-61. DOI: 10.1016/j.ijantimicag.2010.12.001. View

10.

Molla B, Miko A, Pries K, Hildebrandt G, Kleer J, Schroeter A . Class 1 integrons and resistance gene cassettes among multidrug resistant Salmonella serovars isolated from slaughter animals and foods of animal origin in Ethiopia. Acta Trop. 2007; 103(2):142-9. DOI: 10.1016/j.actatropica.2007.05.018. View

11.

Morshed R, Peighambari S . Drug resistance, plasmid profile and random amplified polymorphic DNA analysis of Iranian isolates of Salmonella enteritidis. New Microbiol. 2010; 33(1):47-56. View

12.

Benacer D, Thong K, Watanabe H, Puthucheary S . Characterization of drug resistant Salmonella enterica serotype Typhimurium by antibiograms, plasmids, integrons, resistance genes and PFGE. J Microbiol Biotechnol. 2010; 20(6):1042-52. DOI: 10.4014/jmb.0910.10028. View

13.

Hamidian M, Tajbakhsh M, Walther-Rasmussen J, Zali M . Emergence of extended-spectrum beta-lactamases in clinical isolates of Salmonella enterica in Tehran, Iran. Jpn J Infect Dis. 2009; 62(5):368-71. View

14.

Chu C, Chiu C . Evolution of the virulence plasmids of non-typhoid Salmonella and its association with antimicrobial resistance. Microbes Infect. 2006; 8(7):1931-6. DOI: 10.1016/j.micinf.2005.12.026. View

15.

Ishihara K, Takahashi T, Morioka A, Kojima A, Kijima M, Asai T . National surveillance of Salmonella enterica in food-producing animals in Japan. Acta Vet Scand. 2009; 51:35. PMC: 2743694. DOI: 10.1186/1751-0147-51-35. View

16.

Koeleman J, Stoof J, van der Bijl M, Vandenbroucke-Grauls C, Savelkoul P . Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol. 2001; 39(1):8-13. PMC: 87671. DOI: 10.1128/JCM.39.1.8-13.2001. View

17.

Busani L, Graziani C, Battisti A, Franco A, Ricci A, Vio D . Antibiotic resistance in Salmonella enterica serotypes Typhimurium, Enteritidis and Infantis from human infections, foodstuffs and farm animals in Italy. Epidemiol Infect. 2004; 132(2):245-51. PMC: 2870100. DOI: 10.1017/s0950268803001936. View

18.

Zhao S, Fedorka-Cray P, Friedman S, McDERMOTT P, Walker R, Qaiyumi S . Characterization of Salmonella Typhimurium of animal origin obtained from the National Antimicrobial Resistance Monitoring System. Foodborne Pathog Dis. 2005; 2(2):169-81. DOI: 10.1089/fpd.2005.2.169. View

19.

Vo A, van Duijkeren E, Gaastra W, Fluit A . Antimicrobial resistance, class 1 integrons, and genomic island 1 in Salmonella isolates from Vietnam. PLoS One. 2010; 5(2):e9440. PMC: 2829082. DOI: 10.1371/journal.pone.0009440. View

20.

Naghoni A, Ranjbar R, Tabaraie B, Farshad S, Owlia P, Safiri Z . High prevalence of integron-mediated resistance in clinical isolates of Salmonella enterica. Jpn J Infect Dis. 2010; 63(6):417-21. View