Molecular Characterization of Cryptosporidium Spp. Isolated From Immunocompromised Patients and Children

Overview

Journal Jundishapur J Microbiol

Publisher Brieflands

Specialty Microbiology

Date 2014 Aug 23

PMID 25147696

Citations 13

Authors

Abdollah Rafiei

Zahra Rashno

Alireza Samarbafzadeh

Shahram Khademvatan

Affiliations

Soon will be listed here.

Abstract

Background: Cryptosporidium is known to be one of the most important causes of diarrhea in children and immunocompromised patients. Genotype characterization of Cryptosporidium species in each region would help in the treatment of this disease, as well as to locate the source of infection and to prevent the disease.

Objectives: This current research was conducted in order to analyze the molecular characterization of isolated Cryptosporidium spp. in the Southwest of Iran.

Materials And Methods: In this survey, 390 fecal samples were collected from immunocompromised individuals and children under five-years-of-age. Parasitic infection was evaluated using wet mount preparation, formalin ether, a modified acid fast staining method and microscopic examination. Finally, a PCR-RFLP assay was performed on the extracted DNA collected from fecal samples that were positive for Cryptosporidium by the acid fast method.

Results: Among the 390 fecal samples, 16 cases (4.1%) were infected with Cryptosporidium. Molecular and genotype characterization found the following protozoan species; 11 Cryptosporidium parvum (68.8%), 4 C. hominis (25%), and one case of C. meleagridis (6.2%).

Conclusions: The present study emphasized the public health importance of Cryptosporidium spp. in the study area. In addition, it seems that zoonotic species are the most important causes of infection in the region. As far as we are aware this the first report of a C. meleagridis infection in Iran.

Citing Articles

Multiple protozoal infections in a single immunocompromised patient: A case report.

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The Frequency of Intestinal Parasitic Infections in COVID-19 Patients: A Case-Control Study in Tehran, Capital of Iran.

Taghipour A, Pirestani M, Farahani R, Barati M J Trop Med. 2023; 2023:5359823.

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Spatial Distribution of Common Pathogenic Human Intestinal Protozoa in Iran: A Systematic Review.

Mohebali M, Keshavarz H, Abbaszadeh Afshar M, Hanafi-Bojd A, Hassanpour G Iran J Public Health. 2021; 50(1):69-82.

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Intestinal Parasites Infection in Children with Cancer in Ahvaz, Southwest Iran.

Kahyesh R, Alghasi A, Haddadi S, Sharhani A Interdiscip Perspect Infect Dis. 2021; 2020:8839740.

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Prevalence and Genetic Characterization of Cryptosporidium and Microsporidia Infecting Hematological Malignancy Patients.

Ghoshal U, Kalra S, Tejan N, Ranjan P, Dey A, Nityanand S Acta Parasitol. 2020; 66(2):508-516.

PMID: 33188485 DOI: 10.1007/s11686-020-00307-x.

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