Antimicrobial Activity of Alexidine, Chlorhexidine and Cetrimide Against Streptococcus Mutans Biofilm

Overview

Journal Ann Clin Microbiol Antimicrob

Publisher Biomed Central

Specialties Infectious Diseases
Microbiology

Date 2014 Aug 21

PMID 25139679

Citations 18

Authors

Matilde Ruiz-Linares

Carmen Maria Ferrer-Luque

Teresa Arias-Moliz

Paula de Castro

Beatriz Aguado

Pilar Baca

Affiliations

Soon will be listed here.

Abstract

Background: The use of antimicrobial solutions has been recommended to disinfect demineralized dentin prior to placing the filling material. The aim of this study was to evaluate the ability of several antimicrobials in controlling Streptococcus mutans (SM) biofilm formed in dentin.

Methods: Antimicrobial activity of 0.2% and 2% chlorhexidine (CHX), 0.2% cetrimide (CTR) and 0.2%, 0.5%, 1% and 2% alexidine (ALX) was assayed on 1-week SM biofilm formed on standardized coronal dentin blocks. Results of SM biofilm antimicrobial activity by different protocols were expressed as the kill percentage of biofilm and the term "eradication" was used to denote the kill of 100% of the bacterial population. To compare the efficacies of the different protocols the Student t test was used, previously subjecting data to the Anscombe transformation.

Results: All ALX concentrations tested and 0.2% CTR achieved a kill percentage higher than 99%, followed by 2% CHX with percentages above 96% (no statistically significant difference among them). Whereas 2% ALX and 0.2% CTR respectively showed eradication in 10 and 9 of the twelve specimens, 0.2% CHX did not produce eradication in any case.

Conclusions: The present study shows that, when used for one minute, 2% and 1% alexidine, and 0.2% cetrimide, achieve eradication of Streptococcus mutans biofilm in most specimens when applied to a dentin-volumetric model.

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References

Duque C, Negrini T, Sacono N, Palomari Spolidorio D, de Souza Costa C, Hebling J . Clinical and microbiological performance of resin-modified glass-ionomer liners after incomplete dentine caries removal. Clin Oral Investig. 2009; 13(4):465-71. DOI: 10.1007/s00784-009-0304-2. View

Rosenberg L, Atar M, Daronch M, Honig A, Chey M, Funny M . Observational: prospective study of indirect pulp treatment in primary molars using resin-modified glass ionomer and 2% chlorhexidine gluconate: a 12-month Follow-up. Pediatr Dent. 2013; 35(1):13-7. View

Mohammadi Z, Abbott P . The properties and applications of chlorhexidine in endodontics. Int Endod J. 2009; 42(4):288-302. DOI: 10.1111/j.1365-2591.2008.01540.x. View

Machado Silveira L, Baca P, Arias-Moliz M, Rodriguez-Archilla A, Ferrer-Luque C . Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against Enterococcus faecalis biofilm. Int J Oral Sci. 2013; 5(3):146-9. PMC: 3967334. DOI: 10.1038/ijos.2013.58. View

Weerheijm K, Kreulen C, de Soet J, Groen H, van Amerongen W . Bacterial counts in carious dentine under restorations: 2-year in vivo effects. Caries Res. 1999; 33(2):130-4. DOI: 10.1159/000016506. View

ROBERTS W, Addy M . Comparison of the bisbiguanide antiseptics alexidine and chlorhexidine. I. Effect on plaque accumulation and salivary bacteria. J Clin Periodontol. 1981; 8(3):213-9. DOI: 10.1111/j.1600-051x.1981.tb02032.x. View

Orhan A, Oz F, Ozcelik B, Orhan K . A clinical and microbiological comparative study of deep carious lesion treatment in deciduous and young permanent molars. Clin Oral Investig. 2008; 12(4):369-78. DOI: 10.1007/s00784-008-0208-6. View

Imazato S . Antibacterial properties of resin composites and dentin bonding systems. Dent Mater. 2003; 19(6):449-57. DOI: 10.1016/s0109-5641(02)00102-1. View

Bailon-Sanchez M, Baca P, Ruiz-Linares M, Ferrer-Luque C . Antibacterial and anti-biofilm activity of AH plus with chlorhexidine and cetrimide. J Endod. 2014; 40(7):977-81. DOI: 10.1016/j.joen.2013.11.020. View

10.

Baca P, Junco P, Arias-Moliz M, Castillo F, Rodriguez-Archilla A, Ferrer-Luque C . Antimicrobial substantivity over time of chlorhexidine and cetrimide. J Endod. 2012; 38(7):927-30. DOI: 10.1016/j.joen.2012.04.003. View

11.

Tuzuner T, Kusgoz A, Er K, Tasdemir T, Buruk K, Kemer B . Antibacterial activity and physical properties of conventional glass-ionomer cements containing chlorhexidine diacetate/cetrimide mixtures. J Esthet Restor Dent. 2011; 23(1):46-55. DOI: 10.1111/j.1708-8240.2010.00385.x. View

12.

Cloete T, Jacobs L, Brozel V . The chemical control of biofouling in industrial water systems. Biodegradation. 1998; 9(1):23-37. DOI: 10.1023/a:1008216209206. View

13.

Aas J, Griffen A, Dardis S, Lee A, Olsen I, Dewhirst F . Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol. 2008; 46(4):1407-17. PMC: 2292933. DOI: 10.1128/JCM.01410-07. View

14.

Nikhil V, Madan M, Agarwal C, Suri N . Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine. J Conserv Dent. 2014; 17(3):271-5. PMC: 4056401. DOI: 10.4103/0972-0707.131795. View

15.

Kidd E . How 'clean' must a cavity be before restoration?. Caries Res. 2004; 38(3):305-13. DOI: 10.1159/000077770. View

16.

Botelho M . The antimicrobial activity of a dentin conditioner combined with antibacterial agents. Oper Dent. 2005; 30(1):75-82. View

17.

Simoes M, Pereira M, Vieira M . Effect of mechanical stress on biofilms challenged by different chemicals. Water Res. 2005; 39(20):5142-52. DOI: 10.1016/j.watres.2005.09.028. View

18.

Dame-Teixeira N, Arthur R, Parolo C, Maltz M . Genotypic diversity and virulence traits of Streptococcus mutans isolated from carious dentin after partial caries removal and sealing. ScientificWorldJournal. 2014; 2014:165201. PMC: 3918848. DOI: 10.1155/2014/165201. View

19.

Arias-Moliz M, Ferrer-Luque C, Gonzalez-Rodriguez M, Valderrama M, Baca P . Eradication of Enterococcus faecalis biofilms by cetrimide and chlorhexidine. J Endod. 2009; 36(1):87-90. DOI: 10.1016/j.joen.2009.10.013. View

20.

Bjorndal L, Larsen T . Changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure. Caries Res. 2000; 34(6):502-8. DOI: 10.1159/000016631. View