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Fluorescence-guided Surgery Improves Outcome in an Orthotopic Osteosarcoma Nude-mouse Model

Overview

Overview

Journal J Orthop Res

Publisher Wiley

Specialty Orthopedics

Date 2014 Aug 21

PMID 25138581

Citations 16

Authors

Authors

Shinji Miwa

Yukihiko Hiroshima

Shuya Yano

Yong Zhang

Yasunori Matsumoto

Fuminari Uehara

Mako Yamamoto

Hiroaki Kimura

Katsuhiro Hayashi

Michael Bouvet

Hiroyuki Tsuchiya

Robert M Hoffman

Affiliations

Affiliations

Soon will be listed here.

Abstract

In order to develop a model for fluorescence-guided surgery (FGS), 143B human osteosarcoma cells expressing red fluorescent protein (RFP) were injected into the intramedullary cavity of the tibia in nude mice. The fluorescent areas of residual tumors after bright-light surgery (BLS) and FGS were 10.2 ± 2.4 mm(2) and 0.1 ± 0.1 mm(2) , respectively (p<0.001). The BLS-treated mice and BLS+cisplatinum (CDDP)-treated mice had significant recurrence. In contrast, the FGS mice and FGS+CDDP mice had very little recurring tumor growth. Disease-free survival (DFS) in the BLS-, BLS+CDDP-, FGS-, and FGS+CDDP-treated mice was 12.5%, 37.5%, 75.0%, and 87.5%, respectively. The FGS-treated mice had a significantly higher DFS rate than the BLS-treated mice (p=0.021). The FGS+CDDP-treated mice had significantly higher DFS rate than the BLS+CDDP-treated mice (p=0.043). Although chemotherapy significantly reduced multiple metastases (p=0.033), there was no significant correlation between FGS and lung metastasis. FGS significantly reduced the recurrence of the primary tumor but did not reduce lung metastasis. The combination of FGS and adjuvant CDDP reduced tumor recurrence and prevented multiple metastases. FGS and adjuvant chemotherapy should be performed as early as possible in the disease to prevent both recurrence and metastatic development.

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