Evaluation of Industrial Dairy Waste (milk Dust Powder) for Acetone-butanol-ethanol Production by Solventogenic Clostridium Species

Overview

Journal Springerplus

Date 2014 Aug 16

PMID 25126487

Citations 6

Authors

Victor Ujor

Ashok Kumar Bharathidasan

Katrina Cornish

Thaddeus Chukwuemeka Ezeji

Affiliations

Soon will be listed here.

Abstract

Readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (ABE) fermentation to economic competitiveness. Lactose-replete cheese whey tends to favor the production of butanol over acetone. In the current study, we investigated the fermentability of milk dust powder with high lactose content, for ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Both microorganisms produced 7.3 and 5.8 g/L of butanol respectively, with total ABE concentrations of 10.3 and 8.2 g/L, respectively. Compared to fermentation with glucose, fermentation of milk dust powder increased butanol to acetone ratio by 16% and 36% for C. acetobutylicum and C. beijerinckii, respectively. While these results demonstrate the fermentability of milk dust powder, the physico-chemical properties of milk dust powder appeared to limit sugar utilization, growth and ABE production. Further work aimed at improving the texture of milk dust powder-based medium would likely improve lactose utilization and ABE production.

Citing Articles

Microbial detoxification of lignocellulosic biomass hydrolysates: Biochemical and molecular aspects, challenges, exploits and future perspectives.

Ujor V, Okonkwo C Front Bioeng Biotechnol. 2022; 10:1061667.

PMID: 36483774 PMC: 9723337. DOI: 10.3389/fbioe.2022.1061667.

Validating the preeminence of biochemical properties of camel over cow and goat milk during the Covid-19.

Al-Saffar A Food Sci Nutr. 2022; 10(8):2786-2793.

PMID: 35959268 PMC: 9361446. DOI: 10.1002/fsn3.2881.

Investigating the Processing Potential of Ethiopian Agricultural Residue Enset/ for Biobutanol Production.

Seid N, Griesheimer P, Neumann A Bioengineering (Basel). 2022; 9(4).

PMID: 35447693 PMC: 9025969. DOI: 10.3390/bioengineering9040133.

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives.

Vees C, Neuendorf C, Pflugl S J Ind Microbiol Biotechnol. 2020; 47(9-10):753-787.

PMID: 32894379 PMC: 7658081. DOI: 10.1007/s10295-020-02296-2.

Use of Cupriavidus basilensis-aided bioabatement to enhance fermentation of acid-pretreated biomass hydrolysates by Clostridium beijerinckii.

Agu C, Ujor V, Gopalan V, Ezeji T J Ind Microbiol Biotechnol. 2016; 43(9):1215-26.

PMID: 27400988 DOI: 10.1007/s10295-016-1798-7.

References

Bahl H, Gottwald M, Kuhn A, Rale V, Andersch W, Gottschalk G . Nutritional Factors Affecting the Ratio of Solvents Produced by Clostridium acetobutylicum. Appl Environ Microbiol. 1986; 52(1):169-72. PMC: 203431. DOI: 10.1128/aem.52.1.169-172.1986. View

Qureshi N, Blaschek H . Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation. Appl Biochem Biotechnol. 2000; 84-86:225-35. DOI: 10.1385/abab:84-86:1-9:225. View

Russell J, Diez-Gonzalez F . The effects of fermentation acids on bacterial growth. Adv Microb Physiol. 1997; 39:205-34. DOI: 10.1016/s0065-2911(08)60017-x. View

Sun D, Waters J, Mawhinney T . Determination of thirteen common elements in food samples by inductively coupled plasma atomic emission spectrometry: comparison of five digestion methods. J AOAC Int. 2000; 83(5):1218-24. View

Jesse T, Ezeji T, Qureshi N, Blaschek H . Production of butanol from starch-based waste packing peanuts and agricultural waste. J Ind Microbiol Biotechnol. 2002; 29(3):117-23. DOI: 10.1038/sj.jim.7000285. View

Zhang Y, Han B, Ezeji T . Biotransformation of furfural and 5-hydroxymethyl furfural (HMF) by Clostridium acetobutylicum ATCC 824 during butanol fermentation. N Biotechnol. 2011; 29(3):345-51. DOI: 10.1016/j.nbt.2011.09.001. View

Ezeji T, Milne C, Price N, Blaschek H . Achievements and perspectives to overcome the poor solvent resistance in acetone and butanol-producing microorganisms. Appl Microbiol Biotechnol. 2009; 85(6):1697-712. DOI: 10.1007/s00253-009-2390-0. View

Shi Y, Li Y, Li Y . Large number of phosphotransferase genes in the Clostridium beijerinckii NCIMB 8052 genome and the study on their evolution. BMC Bioinformatics. 2010; 11 Suppl 11:S9. PMC: 3024867. DOI: 10.1186/1471-2105-11-S11-S9. View

Servinsky M, Kiel J, Dupuy N, Sund C . Transcriptional analysis of differential carbohydrate utilization by Clostridium acetobutylicum. Microbiology (Reading). 2010; 156(Pt 11):3478-3491. DOI: 10.1099/mic.0.037085-0. View

10.

Zhang Y, Ezeji T . Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation. Biotechnol Biofuels. 2013; 6(1):66. PMC: 3681630. DOI: 10.1186/1754-6834-6-66. View

11.

Han B, Gopalan V, Ezeji T . Acetone production in solventogenic Clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate. Appl Microbiol Biotechnol. 2011; 91(3):565-76. DOI: 10.1007/s00253-011-3276-5. View

12.

Han B, Ujor V, Lai L, Gopalan V, Ezeji T . Use of proteomic analysis to elucidate the role of calcium in acetone-butanol-ethanol fermentation by Clostridium beijerinckii NCIMB 8052. Appl Environ Microbiol. 2012; 79(1):282-93. PMC: 3536099. DOI: 10.1128/AEM.02969-12. View

13.

Grupe H, Gottschalk G . Physiological Events in Clostridium acetobutylicum during the Shift from Acidogenesis to Solventogenesis in Continuous Culture and Presentation of a Model for Shift Induction. Appl Environ Microbiol. 1992; 58(12):3896-902. PMC: 183201. DOI: 10.1128/aem.58.12.3896-3902.1992. View

14.

Ezeji T, Blaschek H . Fermentation of dried distillers' grains and solubles (DDGS) hydrolysates to solvents and value-added products by solventogenic clostridia. Bioresour Technol. 2007; 99(12):5232-42. DOI: 10.1016/j.biortech.2007.09.032. View

15.

Farrell Jr H, Jimenez-Flores R, Bleck G, Brown E, Butler J, Creamer L . Nomenclature of the proteins of cows' milk--sixth revision. J Dairy Sci. 2004; 87(6):1641-74. DOI: 10.3168/jds.S0022-0302(04)73319-6. View

16.

Nielsen D, Leonard E, Yoon S, Tseng H, Yuan C, Jones Prather K . Engineering alternative butanol production platforms in heterologous bacteria. Metab Eng. 2009; 11(4-5):262-73. DOI: 10.1016/j.ymben.2009.05.003. View

17.

Yu Y, Tangney M, Aass H, Mitchell W . Analysis of the mechanism and regulation of lactose transport and metabolism in Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 2007; 73(6):1842-50. PMC: 1828815. DOI: 10.1128/AEM.02082-06. View

18.

Ren C, Gu Y, Hu S, Wu Y, Wang P, Yang Y . Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum. Metab Eng. 2010; 12(5):446-54. DOI: 10.1016/j.ymben.2010.05.002. View

19.

Thang V, Kanda K, Kobayashi G . Production of acetone-butanol-ethanol (ABE) in direct fermentation of cassava by Clostridium saccharoperbutylacetonicum N1-4. Appl Biochem Biotechnol. 2009; 161(1-8):157-70. DOI: 10.1007/s12010-009-8770-1. View