Butylidenephthalide Blocks Potassium Channels and Enhances Basal Tension in Isolated Guinea-pig Trachea

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Aug 13

PMID 25114927

Citations 5

Authors

Hsin-Te Hsu

You-Lan Yang

Wan-Chen Chen

Chi-Ming Chen

Wun-Chang Ko

Affiliations

Soon will be listed here.

Abstract

Butylidenephthalide (Bdph, 30~300 μM), a constituent of Ligusticum chuanxiong Hort., significantly enhanced tension in isolated guinea-pig trachea. In this study, we investigate the mechanism(s) of Bdph-induced contraction in the tissue. Isolated trachea was bathed in 5 mL of Krebs solution containing indomethacin (3 μM), and its tension changes were isometrically recorded. Cromakalim (3 μM), an ATP-dependent K+ channel opener, significantly antagonized the Bdph-induced enhancement of baseline tension. Bdph (300 μM) also significantly antagonized cromakalim-induced relaxation. Bdph (300 μM) did not significantly influence the antagonistic effects of glibenclamide (GBC, 1 μM) and tetraethylammonium (TEA, 8 mM) against the cromakalim-induced relaxation. However, Bdph (300 μM) and 4-aminopiridine (4-AP, 5 mM), a blocker of K v 1 family of K+ channels, in combination significantly rightward shifted the log concentration-relaxation curve of cromakalim. The antagonistic effect of the combination almost equals the sum of the individual effects of Bdph and 4-AP, suggesting that the antagonistic mechanism of Bdph may be similar to that of 4-AP. All calcium channel blockers influenced neither the baseline tension nor antagonistic effect of Bdph against cromakalim. In conclusion, Bdph may be similar to 4-AP, a blocker of K v 1 family of K+ channels, to enhance the baseline tension of guinea-pig trachea.

Citing Articles

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Mechanisms of butylidenephthalide for twitch facilitation in electrically stimulated mouse vas deferens.

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Lack of effect of Z-butylidenephthalide on presynaptic N-type Ca²⁺ channels in isolated guinea-pig ileum.

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Butylidenephthalide antagonizes cromakalim-induced systolic pressure reduction in conscious normotensive rats.

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