Structural Damage of Chicken Red Blood Cells Exposed to Platinum Nanoparticles and Cisplatin

Overview

Journal Nanoscale Res Lett

Publisher Springer

Specialty Biotechnology

Date 2014 Aug 13

PMID 25114629

Citations 12

Authors

Marta Kutwin

Ewa Sawosz

Slawomir Jaworski

Natalia Kurantowicz

Barbara Strojny

Andre Chwalibog

Affiliations

Soon will be listed here.

Abstract

Side effects and resistance of cancer cells to cisplatin are major drawbacks to its application, and recently, the possibility of replacing cisplatin with nanocompounds has been considered. Most chemotherapeutic agents are administered intravenously, and comparisons between the interactions of platinum nanoparticles (NP-Pt) and cisplatin with blood compartments are important for future applications. This study investigated structural damage, cell membrane deformation and haemolysis of chicken embryo red blood cells (RBC) after treatment with cisplatin and NP-Pt. Cisplatin (4 μg/ml) and NP-Pt (2,6 μg/ml), when incubated with chicken embryo RBC, were detrimental to cell structure and induced haemolysis. The level of haemolytic injury was increased after cisplatin and NP-Pt treatments compared to the control group. Treatment with cisplatin caused structural damage to cell membranes and the appearance of keratocytes, while NP-Pt caused cell membrane deformations (discoid shape of cells was lost) and the formation of knizocytes and echinocytes. This work demonstrated that NP-Pt have potential applications in anticancer therapy, but potential toxic side effects must be explored in future preclinical research.

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