A B-cell-specific Nuclear Protein That Binds to DNA Sites 5' to Immunoglobulin S Alpha Tandem Repeats is Regulated During Differentiation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1989 Dec 1

PMID 2511438

Citations 14

Authors

S H Waters

K U Saikh

J Stavnezer

Affiliations

Soon will be listed here.

Abstract

Immunoglobulin heavy-chain switching is effected by recombination events between sites associated with tandemly repeated switch sequences located 5' to immunoglobulin heavy-chain genes. Using the band mobility shift assay, we have identified two distinct sites 5' to the alpha heavy-chain switch sequence with affinity for a single B-cell-specific DNA-binding protein, S alpha-BP. S alpha-BP was present in nuclear extracts from pre-B and B cells but was not detected in extracts from plasmacytomas, B-cell hybridomas, T-cell lymphomas, or a macrophage cell line. It was also not detectable in other nonlymphoid cells tested. Evidence suggests there are S alpha-BP-binding sites near other immunoglobulin switch sequences. As with the S alpha sites, these sites appear to be distinct from the consensus tandem repeats characteristic of immunoglobulin switch sequences. The possible functions of S alpha-BP on contacting its binding sites are discussed in the context of immunoglobulin heavy-chain switch recombination.

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