Bioengineered Functional Brain-like Cortical Tissue

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Aug 13

PMID 25114234

Citations 135

Authors

Min D Tang-Schomer

James D White

Lee W Tien

L Ian Schmitt

Thomas M Valentin

Daniel J Graziano

Amy M Hopkins

Fiorenzo G Omenetto

Philip G Haydon

David L Kaplan

Affiliations

Soon will be listed here.

Abstract

The brain remains one of the most important but least understood tissues in our body, in part because of its complexity as well as the limitations associated with in vivo studies. Although simpler tissues have yielded to the emerging tools for in vitro 3D tissue cultures, functional brain-like tissues have not. We report the construction of complex functional 3D brain-like cortical tissue, maintained for months in vitro, formed from primary cortical neurons in modular 3D compartmentalized architectures with electrophysiological function. We show that, on injury, this brain-like tissue responds in vitro with biochemical and electrophysiological outcomes that mimic observations in vivo. This modular 3D brain-like tissue is capable of real-time nondestructive assessments, offering previously unidentified directions for studies of brain homeostasis and injury.

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