Responses of a Free-living Benthic Marine Nematode Community to Bioremediation of a PAH Mixture

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2014 Aug 9

PMID 25103943

Citations 1

Authors

Hela Louati

Olfa Ben Said

Amel Soltani

Cristiana Cravo-Laureau

Robert Duran

Patricia Aissa

Ezzeddine Mahmoudi

Olivier Pringault

Affiliations

Soon will be listed here.

Abstract

The objectives of this study were (1) to assess the responses of benthic nematodes to a polycyclic aromatic hydrocarbon (PAH) contamination and (2) to test bioremediation techniques for their efficiency in PAH degradation and their effects on nematodes. Sediments with their natural nematofauna communities from Bizerte lagoon (Tunisia) were subjected to a PAH mixture (100 ppm) of phenanthrene, fluoranthene, and pyrene during 30 days. Nematode abundance and diversity significantly decreased, and the taxonomic structure was altered. Results from multivariate analyses of the species abundance data revealed that PAH treatments were significantly different from the control. Spirinia parasitifera became the dominant species (70 % relative abundance) and appeared to be an "opportunistic" species to PAH contamination while Oncholaimus campylocercoides and Neochromadora peocilosoma were strongly inhibited. Biostimulation (addition of mineral salt medium) and bioaugmentation (inoculation of a hydrocarbonoclastic bacterium) were used as bioremediation techniques. Bioremediation treatments enhanced degradation of all three PAHs, with up to 96 % degradation for phenanthrene resulting in a significant stimulation of nematode abundance relative to control microcosms. Nevertheless, these treatments, especially the biostimulation provoked a weak impact on the community structure and diversity index relative to the control microcosms suggesting their feasibility in biorestoration of contaminated sediments.

Citing Articles

Microbial ecology of hydrocarbon-polluted coastal sediments.

Duran R, Cuny P, Bonin P, Cravo-Laureau C Environ Sci Pollut Res Int. 2015; 22(20):15195-9.

PMID: 26381785 DOI: 10.1007/s11356-015-5373-y.

References

Barata C, Calbet A, Saiz E, Ortiz L, Bayona J . Predicting single and mixture toxicity of petrogenic polycyclic aromatic hydrocarbons to the copepod Oithona davisae. Environ Toxicol Chem. 2006; 24(11):2992-9. DOI: 10.1897/05-189r.1. View

Yu K, Wong A, Yau K, Wong Y, Tam N . Natural attenuation, biostimulation and bioaugmentation on biodegradation of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments. Mar Pollut Bull. 2005; 51(8-12):1071-7. PMC: 2885898. DOI: 10.1016/j.marpolbul.2005.06.006. View

Lindgren J, Hassellov I, Dahllof I . Meiofaunal and bacterial community response to diesel additions in a microcosm study. Mar Pollut Bull. 2012; 64(3):595-601. DOI: 10.1016/j.marpolbul.2011.12.014. View

Mahmoudi E, Essid N, Beyrem H, Hedfi A, Boufahja F, Vitiello P . Effects of hydrocarbon contamination on a free living marine nematode community: results from microcosm experiments. Mar Pollut Bull. 2005; 50(11):1197-204. DOI: 10.1016/j.marpolbul.2005.04.018. View

Boitsov S, Jensen H, Klungsoyr J . Natural background and anthropogenic inputs of polycyclic aromatic hydrocarbons (PAH) in sediments of South-Western Barents Sea. Mar Environ Res. 2009; 68(5):236-45. DOI: 10.1016/j.marenvres.2009.06.013. View

Puente A, Juanes J, Calderon G, Echavarri-Erasun B, Garcia A, Garcia-Castrillo G . Medium-term assessment of the effects of the Prestige oil spill on estuarine benthic communities in Cantabria (Northern Spain, Bay of Biscay). Mar Pollut Bull. 2009; 58(4):487-95. DOI: 10.1016/j.marpolbul.2008.12.010. View

Ramette A . Multivariate analyses in microbial ecology. FEMS Microbiol Ecol. 2007; 62(2):142-60. PMC: 2121141. DOI: 10.1111/j.1574-6941.2007.00375.x. View

Trabelsi S, Driss M . Polycyclic aromatic hydrocarbons in superficial coastal sediments from Bizerte Lagoon, Tunisia. Mar Pollut Bull. 2005; 50(3):344-8. DOI: 10.1016/j.marpolbul.2004.11.031. View

Hedfi A, Boufahja F, Ali M, Aissa P, Mahmoudi E, Beyrem H . Do trace metals (chromium, copper, and nickel) influence toxicity of diesel fuel for free-living marine nematodes?. Environ Sci Pollut Res Int. 2012; 20(6):3760-70. DOI: 10.1007/s11356-012-1305-2. View

10.

Brion D, Pelletier E . Modelling PAHs adsorption and sequestration in freshwater and marine sediments. Chemosphere. 2005; 61(6):867-76. DOI: 10.1016/j.chemosphere.2005.04.097. View

11.

Paisse S, Goni-Urriza M, Stalder T, Stadler T, Budzinski H, Duran R . Ring-hydroxylating dioxygenase (RHD) expression in a microbial community during the early response to oil pollution. FEMS Microbiol Ecol. 2011; 80(1):77-86. DOI: 10.1111/j.1574-6941.2011.01270.x. View

12.

Jacques R, Okeke B, Bento F, Teixeira A, Peralba M, Camargo F . Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. Bioresour Technol. 2007; 99(7):2637-43. DOI: 10.1016/j.biortech.2007.04.047. View

13.

Beyrem H, Mahmoudi E, Essid N, Hedfi A, Boufahja F, Aissa P . Individual and combined effects of cadmium and diesel on a nematode community in a laboratory microcosm experiment. Ecotoxicol Environ Saf. 2007; 68(3):412-8. DOI: 10.1016/j.ecoenv.2006.12.007. View

14.

Miyasaka T, Asami H, Watanabe K . Impacts of bioremediation schemes on bacterial population in naphthalene-contaminated marine sediments. Biodegradation. 2006; 17(3):227-35. DOI: 10.1007/s10532-005-5018-9. View

15.

Neff J, Stout S, Gunster D . Ecological risk assessment of polycyclic aromatic hydrocarbons in sediments: identifying sources and ecological hazard. Integr Environ Assess Manag. 2006; 1(1):22-33. DOI: 10.1897/ieam_2004a-016.1. View

16.

Stauffert M, Duran R, Gassie C, Cravo-Laureau C . Response of archaeal communities to oil spill in bioturbated mudflat sediments. Microb Ecol. 2013; 67(1):108-19. DOI: 10.1007/s00248-013-0288-y. View

17.

Louati H, Ben Said O, Soltani A, Got P, Cravo-Laureau C, Duran R . Biostimulation as an attractive technique to reduce phenanthrene toxicity for meiofauna and bacteria in lagoon sediment. Environ Sci Pollut Res Int. 2013; 21(5):3670-9. DOI: 10.1007/s11356-013-2330-5. View

18.

Louati H, Ben Said O, Soltani A, Got P, Mahmoudi E, Cravo-Laureau C . The roles of biological interactions and pollutant contamination in shaping microbial benthic community structure. Chemosphere. 2013; 93(10):2535-46. DOI: 10.1016/j.chemosphere.2013.09.069. View

19.

Hughes J, Beckles D, Chandra S, Ward C . Utilization of bioremediation processes for the treatment of PAH-contaminated sediments. J Ind Microbiol Biotechnol. 1997; 18(2-3):152-60. DOI: 10.1038/sj.jim.2900308. View

20.

Lei A, Hu Z, Wong Y, Tam N . Removal of fluoranthene and pyrene by different microalgal species. Bioresour Technol. 2006; 98(2):273-80. DOI: 10.1016/j.biortech.2006.01.012. View