Immunogenetics. Chromatin State Dynamics During Blood Formation

Overview

Journal Science

Specialty Science

Date 2014 Aug 9

PMID 25103404

Citations 455

Authors

David Lara-Astiaso

Assaf Weiner

Erika Lorenzo-Vivas

Irina Zaretsky

Diego Adhemar Jaitin

Eyal David

Hadas Keren-Shaul

Alexander Mildner

Deborah Winter

Steffen Jung

Nir Friedman

Ido Amit

Affiliations

Soon will be listed here.

Abstract

Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of homogeneous differentiation intermediates. We developed a high-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation. We identify 48,415 enhancer regions and characterize their dynamics. We find that lineage commitment involves de novo establishment of 17,035 lineage-specific enhancers. These enhancer repertoire expansions foreshadow transcriptional programs in differentiated cells. Combining our enhancer catalog with gene expression profiles, we elucidate the transcription factor network controlling chromatin dynamics and lineage specification in hematopoiesis. Together, our results provide a comprehensive model of chromatin dynamics during development.

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