Validation of an Automated ELISA System for Detection of Antibodies to Aleutian Mink Disease Virus Using Blood Samples Collected in Filter Paper Strips

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2014 Aug 9

PMID 25103400

Citations 12

Authors

Anna Knuuttila

Pirjo Aronen

Majvor Eerola

Ian A Gardner

Anna-Maija K Virtala

Olli Vapalahti

Affiliations

Soon will be listed here.

Abstract

Background: Aleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to validate an AMDV-VP2 -recombinant antigen ELISA, which we developed earlier, in an automated assay format for the detection of anti-AMDV antibodies in mink blood and to determine the accuracy of this test compared with the reference standard (counter-current immunoelectrophoresis, CIEP).

Methods: A blood sampling method based on filter paper 12-strips (blood combs) and a device to introduce these strips to an ELISA plate for elution of the samples were developed. Blood and serum samples were collected from 761 mink from two farms with low (2%) and high (81%) seroprevalences of AMDV infection in 2008. ELISA sensitivity and specificity were estimated with a Bayesian 2-test 2-population model that allowed for conditional dependence between CIEP and ELISA. Agreement between the two tests was assessed with kappa statistic and proportion agreement.

Results: The sensitivity and specificity of the automated ELISA system were estimated to be 96.2% and 98.4%, respectively. Agreement between CIEP and ELISA was high, with a kappa value of 0.976 and overall proportion agreement of 98.8%.

Conclusions: The automated ELISA system combined with blood comb sampling is an accurate test format for the detection of anti-AMDV antibodies in mink blood and offers several advantages, including improved blood sampling and data handling, fast sample throughput time, and reductions in costs and labour inputs.

Citing Articles

A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China.

Yang Z, Li Y, Jiang Y, Wu J, Guan Z, Ge J Front Vet Sci. 2025; 11():1498481.

PMID: 39840339 PMC: 11746015. DOI: 10.3389/fvets.2024.1498481.

Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison).

Vahedi S, Salek Ardestani S, Banabazi M, Fraser Clark K PLoS One. 2024; 19(7):e0306135.

PMID: 39024380 PMC: 11257266. DOI: 10.1371/journal.pone.0306135.

Epidemiology, pathogenesis, and diagnosis of Aleutian disease caused by Aleutian mink disease virus: A literature review with a perspective of genomic breeding for disease control in American mink (Neogale vison).

Vahedi S, Salek Ardestani S, Banabazi M, Clark F Virus Res. 2023; 336:199208.

PMID: 37633597 PMC: 10474236. DOI: 10.1016/j.virusres.2023.199208.

Serum Analytes of American Mink (Neovison Vison) Challenged with Aleutian Mink Disease Virus.

Farid A, Rupasinghe P Animals (Basel). 2022; 12(20).

PMID: 36290111 PMC: 9597810. DOI: 10.3390/ani12202725.

Long-term antibody production and viremia in American mink (Neovison vison) challenged with Aleutian mink disease virus.

Farid A, Hussain I, Rupasinghe P, Stephen J, Arju I BMC Vet Res. 2022; 18(1):364.

PMID: 36192746 PMC: 9531452. DOI: 10.1186/s12917-022-03462-7.

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