Sildenafil Enhances the Peripheral Antinociceptive Effect of Ellagic Acid in the Rat Formalin Test

Overview

Journal Indian J Pharmacol

Specialty Pharmacology

Date 2014 Aug 7

PMID 25097278

Citations 2

Authors

Mohammad Taghi Mansouri

Bahareh Naghizadeh

Behnam Ghorbanzadeh

Affiliations

Soon will be listed here.

Abstract

Objective: Ellagic acid (EA), a major polyphenolic compound of pomegranate juice, produces antinociceptive effects, which are mediated through opioidergic and nitric oxide-cyclic guanosine monophosphate (NO-cGMP) pathways. The present study was conducted to elucidate the peripheral antinociceptive effect of EA alone and in combination with sildenafil in the rat formalin test.

Materials And Methods: Pain was produced by intraplantar injection of formalin (2.5%) in rats and nociceptive behavior was measured as the number of flinches every 5 min in 60 min after injection.

Results: Local administration of EA and sildenafil dose-dependently increased the nociception threshold in both phases of the test. Moreover, sub-effective doses of sildenafil (25 or 50 mcg/paw, i.p.) significantly and dose-dependently enhanced the antinociception induced by a sub-effective dose of EA (60 mcg/paw, i.pl.) in both phases of the test. The antinociception produced by these drugs alone, or in combination, was due to a peripheral site of action, since the administration in the contralateral paw was ineffective.

Conclusion: Our results suggest that EA has local peripheral antinociceptive activity, and enhancement of this effect with sildenafil probably occurs through the inhibition of cGMP metabolism.

Citing Articles

Sildenafil Treatment Eliminates Pruritogenesis and Thermal Hyperalgesia in Rats with Portacaval Shunts.

Belghiti M, Agusti A, Hernandez-Rabaza V, Cabrera-Pastor A, Llansola M, Felipo V Neurochem Res. 2016; 42(3):788-794.

PMID: 27321307 DOI: 10.1007/s11064-016-1980-3.

A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats.

Mansouri M, Hemmati A, Naghizadeh B, Mard S, Rezaie A, Ghorbanzadeh B Indian J Pharmacol. 2015; 47(3):292-8.

PMID: 26069367 PMC: 4450555. DOI: 10.4103/0253-7613.157127.

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