Auxin Perception is Required for Arbuscule Development in Arbuscular Mycorrhizal Symbiosis

Overview

Journal Plant Physiol

Specialty Physiology

Date 2014 Aug 7

PMID 25096975

Citations 61

Authors

Mohammad Etemadi

Caroline Gutjahr

Jean-Malo Couzigou

Mohamed Zouine

Dominique Lauressergues

Antonius Timmers

Corinne Audran

Mondher Bouzayen

Guillaume Becard

Jean-Philippe Combier

Affiliations

Soon will be listed here.

Abstract

Most land plant species live in symbiosis with arbuscular mycorrhizal fungi. These fungi differentiate essential functional structures called arbuscules in root cortical cells from which mineral nutrients are released to the plant. We investigated the role of microRNA393 (miR393), an miRNA that targets several auxin receptors, in arbuscular mycorrhizal root colonization. Expression of the precursors of the miR393 was down-regulated during mycorrhization in three different plant species: Solanum lycopersicum, Medicago truncatula, and Oryza sativa. Treatment of S. lycopersicum, M. truncatula, and O. sativa roots with concentrations of synthetic auxin analogs that did not affect root development stimulated mycorrhization, particularly arbuscule formation. DR5-GUS, a reporter for auxin response, was preferentially expressed in root cells containing arbuscules. Finally, overexpression of miR393 in root tissues resulted in down-regulation of auxin receptor genes (transport inhibitor response1 and auxin-related F box) and underdeveloped arbuscules in all three plant species. These results support the conclusion that miR393 is a negative regulator of arbuscule formation by hampering auxin perception in arbuscule-containing cells.

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