Protein-protein Interactions Between Proteins of Citrus Tristeza Virus Isolates

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2014 Jul 28

PMID 25064367

Authors

Chofong Gilbert Nchongboh

Guan-Wei Wu

Ni Hong

Guo-Ping Wang

Affiliations

Soon will be listed here.

Abstract

Citrus tristeza virus (CTV) is one of the most devastating pathogens of citrus. Its genome is organized into 12 open reading frames (ORFs), of which ten ORFs located at the 3'-terminus of the genome have multiple biological functions. The ten genes at the 3'-terminus of the genome of a severe isolate (CTV-S4) and three ORFs (CP, CPm and p20) of three other isolates (N4, S45 and HB1) were cloned into pGBKT7 and pGADT7 yeast shuttle vectors. Yeast two-hybridization (Y2H) assays results revealed a strong self-interaction for CP and p20, and a unique interaction between the CPm of CTV-S4 (severe) and CP of CTV-N4 (mild) isolates. Bimolecular fluorescence complementation also confirmed these interactions. Analysis of the deletion mutants delineated the domains of CP and p20 self-interaction. Furthermore, the domains responsible for CP and p20 self-interactions were mapped at the CP amino acids sites 41-84 and p20 amino acids sites 1-21 by Y2H. This study provided new information on CTV protein interactions which will help for further understanding the biological functions.

Citing Articles

Citrus tristeza virus p20 suppresses antiviral RNA silencing by co-opting autophagy-related protein 8 to mediate the autophagic degradation of SGS3.

Zhang Y, Yang Z, Zhang Z, Wang G, Li X, Hong N PLoS Pathog. 2025; 21(2):e1012960.

PMID: 39993018 PMC: 11882097. DOI: 10.1371/journal.ppat.1012960.

References

Stewart L, Hwang M, Falk B . Two Crinivirus-specific proteins of Lettuce infectious yellows virus (LIYV), P26 and P9, are self-interacting. Virus Res. 2009; 145(2):293-9. DOI: 10.1016/j.virusres.2009.07.021. View

Tyagi M, Thangudu R, Zhang D, Bryant S, Madej T, Panchenko A . Homology inference of protein-protein interactions via conserved binding sites. PLoS One. 2012; 7(1):e28896. PMC: 3269416. DOI: 10.1371/journal.pone.0028896. View

Dimitrova M, Imbert I, Kieny M, Schuster C . Protein-protein interactions between hepatitis C virus nonstructural proteins. J Virol. 2003; 77(9):5401-14. PMC: 153952. DOI: 10.1128/jvi.77.9.5401-5414.2003. View

Littler S, Hubbard S . Conservation of orientation and sequence in protein domain--domain interactions. J Mol Biol. 2005; 345(5):1265-79. DOI: 10.1016/j.jmb.2004.11.011. View

Ruiz-Ruiz S, Soler N, Sanchez-Navarro J, Fagoaga C, Lopez C, Navarro L . Citrus tristeza virus p23: determinants for nucleolar localization and their influence on suppression of RNA silencing and pathogenesis. Mol Plant Microbe Interact. 2013; 26(3):306-18. DOI: 10.1094/MPMI-08-12-0201-R. View

Karasev A . Genetic Diversity and Evolution of Closteroviruses. Annu Rev Phytopathol. 2001; 38:293-324. DOI: 10.1146/annurev.phyto.38.1.293. View

Folimonova S, Robertson C, Shilts T, Folimonov A, Hilf M, Garnsey S . Infection with strains of Citrus tristeza virus does not exclude superinfection by other strains of the virus. J Virol. 2009; 84(3):1314-25. PMC: 2812332. DOI: 10.1128/JVI.02075-09. View

Gowda S, Tatineni S, Folimonova S, Hilf M, Dawson W . Accumulation of a 5' proximal subgenomic RNA of Citrus tristeza virus is correlated with encapsidation by the minor coat protein. Virology. 2009; 389(1-2):122-31. DOI: 10.1016/j.virol.2009.04.009. View

Haas M, Geldreich A, Bureau M, Dupuis L, Leh V, Vetter G . The open reading frame VI product of Cauliflower mosaic virus is a nucleocytoplasmic protein: its N terminus mediates its nuclear export and formation of electron-dense viroplasms. Plant Cell. 2005; 17(3):927-43. PMC: 1069709. DOI: 10.1105/tpc.104.029017. View

10.

Karasev A, Boyko V, Gowda S, Nikolaeva O, Hilf M, Koonin E . Complete sequence of the citrus tristeza virus RNA genome. Virology. 1995; 208(2):511-20. DOI: 10.1006/viro.1995.1182. View

11.

Lopez L, Urzainqui A, Dominguez E, Garcia J . Identification of an N-terminal domain of the plum pox potyvirus CI RNA helicase involved in self-interaction in a yeast two-hybrid system. J Gen Virol. 2001; 82(Pt 3):677-686. DOI: 10.1099/0022-1317-82-3-677. View

12.

Lu R, Folimonov A, Shintaku M, Li W, Falk B, Dawson W . Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci U S A. 2004; 101(44):15742-7. PMC: 524217. DOI: 10.1073/pnas.0404940101. View

13.

Zhang X, Zhao X, Zhang Y, Niu S, Qu F, Zhang Y . N-terminal basic amino acid residues of Beet black scorch virus capsid protein play a critical role in virion assembly and systemic movement. Virol J. 2013; 10:200. PMC: 3691604. DOI: 10.1186/1743-422X-10-200. View

14.

Persico M, Ceol A, Gavrila C, Hoffmann R, Florio A, Cesareni G . HomoMINT: an inferred human network based on orthology mapping of protein interactions discovered in model organisms. BMC Bioinformatics. 2005; 6 Suppl 4:S21. PMC: 1866386. DOI: 10.1186/1471-2105-6-S4-S21. View

15.

Folimonova S . Superinfection exclusion is an active virus-controlled function that requires a specific viral protein. J Virol. 2012; 86(10):5554-61. PMC: 3347309. DOI: 10.1128/JVI.00310-12. View

16.

Harper S, Dawson T, Pearson M . Isolates of Citrus tristeza virus that overcome Poncirus trifoliata resistance comprise a novel strain. Arch Virol. 2010; 155(4):471-80. DOI: 10.1007/s00705-010-0604-5. View

17.

Satyanarayana T, Gowda S, Boyko V, Albiach-Marti M, Mawassi M, Navas-Castillo J . An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication. Proc Natl Acad Sci U S A. 1999; 96(13):7433-8. PMC: 22103. DOI: 10.1073/pnas.96.13.7433. View

18.

Satyanarayana T, Gowda S, Ayllon M, Albiach-Marti M, Dawson W . Mutational analysis of the replication signals in the 3'-nontranslated region of citrus tristeza virus. Virology. 2002; 300(1):140-52. DOI: 10.1006/viro.2002.1550. View

19.

Klaassen V, Mayhew D, Fisher D, Falk B . In vitro transcripts from cloned cDNAs of the lettuce infectious yellows closterovirus bipartite genomic RNAs are competent for replication in Nicotiana benthamiana protoplasts. Virology. 1996; 222(1):169-75. DOI: 10.1006/viro.1996.0407. View

20.

Yang Z, Mathews D, Dodds J, Mirkov T . Molecular characterization of an isolate of citrus tristeza virus that causes severe symptoms in sweet orange. Virus Genes. 1999; 19(2):131-42. DOI: 10.1023/a:1008127224147. View