Facile Enzymatic Synthesis of Base J-containing Oligodeoxyribonucleotides and an Analysis of the Impact of Base J on DNA Replication in Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jul 26

PMID 25061973

Citations 3

Authors

Debin Ji

Yinsheng Wang

Affiliations

Soon will be listed here.

Abstract

We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU) in both single- and double-stranded ODNs, though the latter reaction occurred only when 5hmU was mispaired with a guanine. In addition, base J blocked moderately DNA replication, but it did not induce mutations during replication in human cells.

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