Leptin Modulates Autophagy in Human CD4+CD25- Conventional T Cells

Overview

Journal Metabolism

Specialty Endocrinology

Date 2014 Jul 26

PMID 25060689

Citations 23

Authors

Silvana Cassano

Valentina Pucino

Claudia La Rocca

Claudio Procaccini

Veronica De Rosa

Gianni Marone

Giuseppe Matarese

Affiliations

Soon will be listed here.

Abstract

Objective: In this report we show that the adipocytokine leptin directly modulates autophagy in human CD4(+)CD25(-) conventional (Tconv) T cells.

Results: In vitro treatment with recombinant human leptin determined an inhibition of autophagy during T cell receptor (TCR) stimulation, and this phenomenon was dose- and time-dependent. The events were secondary to the activation of the mammalian-target of rapamycin (mTOR)-pathway induced by leptin, as testified by its reversion induced by mTOR inhibition with rapamycin. At molecular level these phenomena associated with Bcl-2 up-regulation and its interaction with Beclin-1, whose complex exerts a negative effect on autophagy.

Materials/methods: The impact of leptin on autophagy of Tconv cells was determined at biochemical level by western blotting and by flow cytometry; the interaction between BCL-2 and Beclin-1 by co-immunoprecipitation assays.

Conclusions: Our results, suggest that in unconditioned, freshly-isolated human Tconv cells, autophagy and proliferation are controlled by leptin during TCR-engagement, and that both phenomena occur alternatively indicating a balance between these processes during immune activation.

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