Construction, Stable Transformation, and Function of an Amber Suppressor TRNA Gene in Drosophila Melanogaster

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Sep 1

PMID 2505255

Citations 22

Authors

F A Laski

S Ganguly

P A Sharp

U L RajBhandary

G M Rubin

Affiliations

Soon will be listed here.

Abstract

Drosophila melanogaster strains with a stably incorporated amber suppressor tRNA gene have been generated. A tRNATyr gene was site specifically mutated to produce an anticodon sequence that recognizes the amber codon and then introduced into Drosophila by using P-element-mediated transformation. Transformants from four integration events were recovered. Two integrations resulted in both male and female sterility, whereas the other two resulted in male sterility but female fertility. Strains derived from the two female-fertile integration events were shown to have a low level of amber-suppressing activity by their ability to suppress an amber mutation in a chloramphenicol acetyltransferase gene.

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