Real-time Imaging of the Epithelial-mesenchymal Transition Using MicroRNA-200a Sequence-based Molecular Beacon-conjugated Magnetic Nanoparticles

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jul 23

PMID 25048580

Citations 8

Authors

Yoonseok Choi

Hoe Suk Kim

Jisu Woo

Eun Hye Hwang

Kyoung-Won Cho

Soonhag Kim

Woo Kyung Moon

Affiliations

Soon will be listed here.

Abstract

The epithelial-mesenchymal transition (EMT) plays important roles in tumor progression to metastasis. Thus, the development of an imaging probe that can monitor transient periods of the EMT process in live cells is required for a better understanding of metastatic process. Inspired by the fact that the mRNA expression levels of zinc finger E-box-binding homeobox 1 (ZEB1) increase when cells adopt mesenchyme characteristics and that microRNA-200a (miR-200a) can bind to ZEB1 mRNA, we conjugated molecular beacon (MB) mimicking mature miR-200a to magnetic nanoparticles (miR-200a-MB-MNPs) and devised an imaging method to observe transitional changes in the cells during EMT. Transforming growth factor-β1 treated epithelial cells and breast cancer cell lines representing both epithelial and mesenchymal phenotypes were used for the validation of miR-200a-MB-MNPs as an EMT imaging probe. The real-time imaging of live cells acquired with the induction of EMT revealed an increase in fluorescence signals by miR-200a-MB-MNPs, cell morphology alterations, and the loss of cell-cell adhesion. Our results suggest that miR-200a-MB-MNPs can be used as an imaging probe for the real-time monitoring of the EMT process in live cells.

Citing Articles

Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment-colorectal cancer network.

Ieda T, Tazawa H, Okabayashi H, Yano S, Shigeyasu K, Kuroda S Sci Rep. 2019; 9(1):16378.

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Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging.

Chen Z, Peng Y, Xie X, Feng Y, Li T, Li S Theranostics. 2019; 9(20):5784-5796.

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EMT in cancer.

Brabletz T, Kalluri R, Nieto M, Weinberg R Nat Rev Cancer. 2018; 18(2):128-134.

PMID: 29326430 DOI: 10.1038/nrc.2017.118.

Dual Functional Roles of Molecular Beacon as a MicroRNA Detector and Inhibitor.

Li W, Chan C, Miller A, Lee C J Biol Chem. 2017; 292(9):3568-3580.

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In Vivo Visualization and Characterization of Epithelial-Mesenchymal Transition in Breast Tumors.

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