Molecular Analysis of Lipooligosaccharide Biosynthesis in Neisseria Gonorrhoeae

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1989 Sep 1

PMID 2503447

Citations 13

Authors

E F Petricoin 3rd

D C Stein

Affiliations

Soon will be listed here.

Abstract

A HindIII gene bank of Neisseria gonorrhoeae MUG116 was constructed in the cosmid vector pHC79. A cosmid (pSY81) was isolated that was able to convert N. gonorrhoeae FA5100 to reactivity with monoclonal antibody (MAb) 2-1-L8. Several MAb-reactive transformants were isolated and characterized with respect to lipooligosaccharide (LOS) production as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, their ability to react with two other LOS-specific MAbs (3F11 and O6B4), and Southern blot analysis. Escherichia coli containing the clone had altered lipopolysaccharide expression as determined by electrophoretic analysis; however, no reactivity was seen with gonococcus-specific MAbs. The introduction of pSY81 into FA5100 had a pleiomorphic effect, giving rise to transformants having the full parental phenotype or transformants lacking reactivity to a combination of LOS-specific MAbs. Southern blot analysis indicated that the LOS biosynthetic mutation in FA5100 was not due to chromosomal rearrangement or large deletions.

Citing Articles

Sequence-based predictions of lipooligosaccharide diversity in the Neisseriaceae and their implication in pathogenicity.

Stein D, Miller C, Bhoopalan S, Sommer D PLoS One. 2011; 6(4):e18923.

PMID: 21533118 PMC: 3078933. DOI: 10.1371/journal.pone.0018923.

Analysis of lipooligosaccharide biosynthesis in the Neisseriaceae.

Arking D, Tong Y, Stein D J Bacteriol. 2001; 183(3):934-41.

PMID: 11208792 PMC: 94961. DOI: 10.1128/JB.183.3.934-941.2001.

Effect of alpha-oligosaccharide phenotype of Neisseria gonorrhoeae strain MS11 on invasion of Chang conjunctival, HEC-1-B endometrial, and ME-180 cervical cells.

Minor S, Banerjee A, Gotschlich E Infect Immun. 2000; 68(12):6526-34.

PMID: 11083761 PMC: 97746. DOI: 10.1128/IAI.68.12.6526-6534.2000.

Role of lipooligosaccharide in Opa-independent invasion of Neisseria gonorrhoeae into human epithelial cells.

Song W, Ma L, Chen R, Stein D J Exp Med. 2000; 191(6):949-60.

PMID: 10727457 PMC: 2193109. DOI: 10.1084/jem.191.6.949.

Identification of the gene (lgtG) encoding the lipooligosaccharide beta chain synthesizing glucosyl transferase from Neisseria gonorrhoeae.

Banerjee A, Wang R, Uljon S, Rice P, Gotschlich E, Stein D Proc Natl Acad Sci U S A. 1998; 95(18):10872-7.

PMID: 9724797 PMC: 27988. DOI: 10.1073/pnas.95.18.10872.

References

Morse S, Vaughan P, Johnson D, Iglewski B . Inhibition of Neisseria gonorrhoeae by a bacteriocin from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976; 10(2):354-62. PMC: 429747. DOI: 10.1128/AAC.10.2.354. View

Chomczynski P, Qasba P . Alkaline transfer of DNA to plastic membrane. Biochem Biophys Res Commun. 1984; 122(1):340-4. DOI: 10.1016/0006-291x(84)90480-7. View

Creeger E, Chen J, Rothfield L . Cloning of genes for bacterial glycosyltransferases. II. Selection of a hybrid plasmid carrying the rfah gene. J Biol Chem. 1979; 254(3):811-5. View

Hohn B, Collins J . A small cosmid for efficient cloning of large DNA fragments. Gene. 1980; 11(3-4):291-8. DOI: 10.1016/0378-1119(80)90069-4. View

Gregg C, MELLY M, Hellerqvist C, CONIGLIO J, McGee Z . Toxic activity of purified lipopolysaccharide of Neisseria gonorrhoeae for human fallopian tube mucosa. J Infect Dis. 1981; 143(3):432-9. DOI: 10.1093/infdis/143.3.432. View

Connelly M, Stein D, Young F, Morse S, Allen P . Interaction with lectins and differential wheat germ agglutinin binding of pyocin 103-sensitive and -resistant Neisseria gonorrhoeae. J Bacteriol. 1981; 148(3):796-803. PMC: 216277. DOI: 10.1128/jb.148.3.796-803.1981. View

Morse S, Apicella M . Isolation of a lipopolysaccharide mutant of Neisseria gonorrhoeae: an analysis of the antigenic and biologic difference. J Infect Dis. 1982; 145(2):206-16. DOI: 10.1093/infdis/145.2.206. View

Tsai C, Frasch C . A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982; 119(1):115-9. DOI: 10.1016/0003-2697(82)90673-x. View

Schneider H, Griffiss J, Williams G, Pier G . Immunological basis of serum resistance of Neisseria gonorrhoeae. J Gen Microbiol. 1982; 128(1):13-22. DOI: 10.1099/00221287-128-1-13. View

10.

Hitchcock P, Brown T . Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983; 154(1):269-77. PMC: 217456. DOI: 10.1128/jb.154.1.269-277.1983. View

11.

Stein D, Young F, Tenover F, Clark V . Characterization of a chimeric beta-lactamase plasmid of Neisseria gonorrhoeae which can function in Escherichia coli. Mol Gen Genet. 1983; 189(1):77-84. DOI: 10.1007/BF00326058. View

12.

Shafer W, Joiner K, Guymon L, Cohen M, Sparling P . Serum sensitivity of Neisseria gonorrhoeae: the role of lipopolysaccharide. J Infect Dis. 1984; 149(2):175-83. DOI: 10.1093/infdis/149.2.175. View

13.

Church G, Gilbert W . Genomic sequencing. Proc Natl Acad Sci U S A. 1984; 81(7):1991-5. PMC: 345422. DOI: 10.1073/pnas.81.7.1991. View

14.

Schneider H, Hale T, Zollinger W, Seid Jr R, Hammack C, Griffiss J . Heterogeneity of molecular size and antigenic expression within lipooligosaccharides of individual strains of Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 1984; 45(3):544-9. PMC: 263327. DOI: 10.1128/iai.45.3.544-549.1984. View

15.

Sidberry H, Kaufman B, Wright D, Sadoff J . Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose. J Immunol Methods. 1985; 76(2):299-305. DOI: 10.1016/0022-1759(85)90307-2. View

16.

Schneider H, Griffiss J, Mandrell R, Jarvis G . Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae. Infect Immun. 1985; 50(3):672-7. PMC: 261131. DOI: 10.1128/iai.50.3.672-677.1985. View

17.

Sparling P, Cannon J, So M . Phase and antigenic variation of pili and outer membrane protein II of Neisseria gonorrhoeae. J Infect Dis. 1986; 153(2):196-201. DOI: 10.1093/infdis/153.2.196. View

18.

Rehemtulla A, Kadam S, Sanderson K . Cloning and analysis of the sfrB (sex factor repression) gene of Escherichia coli K-12. J Bacteriol. 1986; 166(2):651-7. PMC: 214654. DOI: 10.1128/jb.166.2.651-657.1986. View

19.

Mandrell R, Schneider H, Apicella M, Zollinger W, Rice P, Griffiss J . Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides. Infect Immun. 1986; 54(1):63-9. PMC: 260117. DOI: 10.1128/iai.54.1.63-69.1986. View

20.

Apicella M, Shero M, Jarvis G, Griffiss J, Mandrell R, Schneider H . Phenotypic variation in epitope expression of the Neisseria gonorrhoeae lipooligosaccharide. Infect Immun. 1987; 55(8):1755-61. PMC: 260597. DOI: 10.1128/iai.55.8.1755-1761.1987. View