A Rabbit Model of Non-typhoidal Salmonella Bacteremia

Overview

Journal Comp Immunol Microbiol Infect Dis

Specialties Allergy & Immunology
Infectious Diseases
Microbiology
Veterinary Medicine

Date 2014 Jul 19

PMID 25033732

Citations 3

Authors

Aruna Panda

Ivan Tatarov

Billie Jo Masek

Justin Hardick

Annabelle Crusan

Teresa Wakefield

Karen Carroll

Samuel Yang

Yu-Hsiang Hsieh

Michael M Lipsky

Charles G McLeod

Myron M Levine

Richard E Rothman

Charlotte A Gaydos

Louis J DeTolla

Affiliations

Soon will be listed here.

Abstract

Bacteremia is an important cause of morbidity and mortality in humans. In this study, we focused on the development of an animal model of bacteremia induced by non-typhoidal Salmonella. New Zealand White rabbits were inoculated with a human isolate of non-typhoidal Salmonella strain CVD J73 via the intra-peritoneal route. Blood samples were collected at specific time points and at euthanasia from infected rabbits. Additionally, tissue samples from the heart, lungs, spleen, gastrointestinal tract, liver and kidneys were obtained at euthanasia. All experimentally infected rabbits displayed clinical signs of disease (fever, dehydration, weight loss and lethargy). Tissues collected at necropsy from the animals exhibited histopathological changes indicative of bacteremia. Non-typhoidal Salmonella bacteria were detected in the blood and tissue samples of infected rabbits by microbiological culture and real-time PCR assays. The development of this animal model of bacteremia could prove to be a useful tool for studying how non-typhoidal Salmonella infections disseminate and spread in humans.

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