A Novel Recombinant BCG Vaccine Encoding Eimeria Tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2014 Jul 18

PMID 25031464

Citations 12

Authors

Qiuyue Wang

Lifeng Chen

Jianhua Li

Jun Zheng

Ning Cai

Pengtao Gong

Shuhong Li

He Li

Xichen Zhang

Affiliations

Soon will be listed here.

Abstract

A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4(+) and CD8(+) cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.

Citing Articles

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