The 5S RNA Gene Minichromosome of Euplotes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Jun 26

PMID 2501759

Citations 5

Authors

A E Roberson

A P Wolffe

L J Hauser

D E Olins

Affiliations

Soon will be listed here.

Abstract

The macronucleus of the ciliated protozoan Euplotes eurystomus contains about 10(6) copies of a single type of 5S ribosomal RNA gene. This 5S gene DNA is only 930 bp long, is flanked by telomeres, and contains a single coding region of 120 bp which serves as a template for transcription in vivo and in vitro. The 5S gene minichromatin possesses four positioned nucleosomes and hypersensitive cleavage sites in the telomeric regions.

Citing Articles

Comparative analyses among the Trichomonas vaginalis, Trichomonas tenax, and Tritrichomonas foetus 5S ribosomal RNA genes.

Torres-Machorro A, Hernandez R, Alderete J, Lopez-Villasenor I Curr Genet. 2009; 55(2):199-210.

PMID: 19290527 DOI: 10.1007/s00294-009-0237-y.

A unique pattern of intrastrand anomalies in base composition of the DNA in hypotrichs.

Prescott D, Dizick S Nucleic Acids Res. 2000; 28(23):4679-88.

PMID: 11095678 PMC: 115164. DOI: 10.1093/nar/28.23.4679.

Macronuclear gene-sized molecules of hypotrichs.

Hoffman D, Anderson R, DuBois M, Prescott D Nucleic Acids Res. 1995; 23(8):1279-83.

PMID: 7753617 PMC: 306850. DOI: 10.1093/nar/23.8.1279.

Inaccessibility of the Euplotes telomere binding protein.

Olins A, Cacheiro L, Herrmann A, Dhar M, Olins D Chromosoma. 1993; 102(10):700-11.

PMID: 7512014 DOI: 10.1007/BF00650896.

Structure of the macronuclear polyubiquitin gene in Euplotes.

Hauser L, Roberson A, Olins D Chromosoma. 1991; 100(6):386-94.

PMID: 1654239 DOI: 10.1007/BF00337517.

References

Lipps H, Erhardt P . DNase I hypersensitivity of the terminal inverted repeat DNA sequences in the macronucleus of the ciliate Stylonychia mytilus. FEBS Lett. 1981; 126(2):219-22. DOI: 10.1016/0014-5793(81)80246-3. View

Klobutcher L, Swanton M, Donini P, Prescott D . All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus. Proc Natl Acad Sci U S A. 1981; 78(5):3015-9. PMC: 319490. DOI: 10.1073/pnas.78.5.3015. View

Soeda E, Miura K, Nakaso A, Kimura G . Nucleotide sequence around the replication origin of polyoma virus DNA. FEBS Lett. 1977; 79(2):383-9. DOI: 10.1016/0014-5793(77)80826-0. View

Birkenmeier E, Brown D, Jordan E . A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. Cell. 1978; 15(3):1077-86. DOI: 10.1016/0092-8674(78)90291-x. View

Lipps H, Steinbruck G . Free genes for rRNAs in the macronuclear genome of the ciliate Stylonychia mytilus. Chromosoma. 1978; 69(1):21-6. DOI: 10.1007/BF00327378. View

Rae P, Spear B . Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax. Proc Natl Acad Sci U S A. 1978; 75(10):4992-6. PMC: 336248. DOI: 10.1073/pnas.75.10.4992. View

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

Sakonju S, Bogenhagen D, Brown D . A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. Cell. 1980; 19(1):13-25. DOI: 10.1016/0092-8674(80)90384-0. View

Wormington W, Brown D . Onset of 5 S RNA gene regulation during Xenopus embryogenesis. Dev Biol. 1983; 99(1):248-57. DOI: 10.1016/0012-1606(83)90273-7. View

10.

Murti K, Prescott D . Replication forms of the gene-sized DNA molecules of hypotrichous ciliates. Mol Cell Biol. 1983; 3(9):1562-6. PMC: 370009. DOI: 10.1128/mcb.3.9.1562-1566.1983. View

11.

Kumazaki T, Hori H, Osawa S . Phylogeny of protozoa deduced from 5S rRNA sequences. J Mol Evol. 1983; 19(6):411-9. DOI: 10.1007/BF02102316. View

12.

Montiel J, Norbury C, Tuite M, Dobson M, MILLS J, Kingsman A . Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae. Nucleic Acids Res. 1984; 12(2):1049-68. PMC: 318555. DOI: 10.1093/nar/12.2.1049. View

13.

Pederson D, Yao M, Kimmel A, Gorovsky M . Sequence organization within and flanking clusters of 5S ribosomal RNA genes in Tetrahymena. Nucleic Acids Res. 1984; 12(6):3003-21. PMC: 318722. DOI: 10.1093/nar/12.6.3003. View

14.

Ladiges W, Raff R, Brown S, Deeg H, Storb R . The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT). Immunogenetics. 1984; 19(4):359-65. DOI: 10.1007/BF00345410. View

15.

Schlissel M, Brown D . The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1. Cell. 1984; 37(3):903-13. DOI: 10.1016/0092-8674(84)90425-2. View

16.

Gottschling D, Cech T . Chromatin structure of the molecular ends of Oxytricha macronuclear DNA: phased nucleosomes and a telomeric complex. Cell. 1984; 38(2):501-10. DOI: 10.1016/0092-8674(84)90505-1. View

17.

Pederson D, Shupe K, Gorovsky M . Changes in chromatin structure accompany modulation of the rate of transcription of 5S ribosomal genes in Tetrahymena. Nucleic Acids Res. 1984; 12(22):8489-507. PMC: 320393. DOI: 10.1093/nar/12.22.8489. View

18.

Bieker J, Martin P, Roeder R . Formation of a rate-limiting intermediate in 5S RNA gene transcription. Cell. 1985; 40(1):119-27. DOI: 10.1016/0092-8674(85)90315-0. View

19.

Cartwright I, Elgin S . Chemical footprinting of 5S RNA chromatin in embryos of Drosophila melanogaster. EMBO J. 1984; 3(13):3101-8. PMC: 557824. DOI: 10.1002/j.1460-2075.1984.tb02265.x. View

20.

Setzer D, Brown D . Formation and stability of the 5 S RNA transcription complex. J Biol Chem. 1985; 260(4):2483-92. View