Establishment of a Xeno-free Culture System That Preserves the Characteristics of Placenta Mesenchymal Stem Cells

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2014 Jul 7

PMID 24997581

Citations 1

Authors

Saeyoung Park

Hye-Ran Park

Won-Don Lee

Chang-Young Hur

Young-Jay Lee

Affiliations

Soon will be listed here.

Abstract

Although stem cells are promising candidates for cell replacement therapies, the vast majority are derived using animal sera, which has risk of being contaminated by animal viruses or toxins. To overcome these potential problems, we initially established multiple lines of stem cells from first-trimester human placenta (fPMSC), which were cultivated using human follicular fluid (hFF) instead of fetal bovine serum (FBS). FF provides a very important microenvironment for the development of oocytes. No differences were found in the general morphology, growth rate, karyotype, gene and surface expressions between placental MSCs cultured in 5 % hFF-supplemented medium (fPMSC-X) or 10 % FBS-supplemented medium (fPMSC). Differentiation experiments confirmed similar levels of potency in cells grown in either condition. Since hFF preserved the unique features of the stem cells and is free from potential pathogens, it should be considered as the main culture medium supplement for the propagation of human stem cells for clinical applications.

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