Effect of Maternal Protein Restriction on Liver Metabolism in Rat Offspring

Overview

Journal J Physiol Sci

Specialty Physiology

Date 2014 Jul 5

PMID 24994532

Citations 11

Authors

Camila Moraes

Hercules J Rebelato

Maria Esmeria C Amaral

Thais Marangoni Resende

Eduarda V C Silva

Marcelo A M Esquisatto

Rosana Catisti

Affiliations

Soon will be listed here.

Abstract

Consequences of gestational protein restriction (GPR) on liver metabolism in rat offspring were investigated. Pregnant dams were divided into groups: normal (NP, 17% casein) or low-protein diet (LP, 6% casein). Livers were collected from 30-day-old offspring (d30) for analysis or isolation of mitochondria. At d30, hepatic and muscle glycogen was increased in LP group. Mitochondrial swelling and oxygen uptake (recorded with a Clark-type electrode) were significantly reduced in NP female and LP pups. Thiobarbituric acid reactive substances production was lower in females (NP or LP), suggesting significant inhibition of lipid peroxidation. Measurement of mitochondrial respiration (states 3 and 4 stimulated by succinate) showed a higher ADP/O ratio in LP pups, particularly females, suggesting higher phosphorylation efficiency. In the 1st month of life, under our experimental conditions, GPR protects liver mitochondria against oxidative stress and females seem to be more resistant or more suitable for survival.

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