CXC Chemokine Ligand 12 Protects Pancreatic β-cells from Necrosis Through Akt Kinase-mediated Modulation of Poly(ADP-ribose) Polymerase-1 Activity

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jul 3

PMID 24988468

Citations 5

Authors

Nevena Grdovic

Svetlana Dinic

Mirjana Mihailovic

Aleksandra Uskokovic

Jelena Arambasic Jovanovic

Goran Poznanovic

Ludwig Wagner

Melita Vidakovic

Affiliations

Soon will be listed here.

Abstract

The diabetes prevention paradigm envisages the application of strategies that support the maintenance of appropriate β-cell numbers. Herein we show that overexpression of CXC chemokine ligand12 (CXCL12) considerably improves the viability of isolated rat Langerhans islet cells and Rin-5F pancreatic β-cells after hydrogen peroxide treatment. In rat islets and wt cells hydrogen peroxide treatment induced necrotic cell death that was mediated by the rapid and extensive activation of poly(ADP-ribose) polymerase-1 (PARP-1). In contrast, CXCL12-overexpressing cells were protected from necrotic cell death as a result of significantly reduced PARP-1 activity. CXCL12 downstream signalling through Akt kinase was responsible for the reduction of PARP-1 activity which switched cell death from necrosis to apoptosis, providing increased protection to cells from oxidative stress. Our results offer a novel aspect of the CXCL12-mediated improvement of β-cell viability which is based on its antinecrotic action through modulation of PARP-1 activity.

Citing Articles

Protective Effect of CXCR4 Antagonist DBPR807 against Ischemia-Reperfusion Injury in a Rat and Porcine Model of Myocardial Infarction: Potential Adjunctive Therapy for Percutaneous Coronary Intervention.

Yeh K, Lee C, Song J, Wu C, Yeh T, Wu S Int J Mol Sci. 2022; 23(19).

PMID: 36233031 PMC: 9570210. DOI: 10.3390/ijms231911730.

SDF-1 inhibits the dedifferentiation of islet β cells in hyperglycaemia by up-regulating FoxO1 via binding to CXCR4.

Chen X, Shi Y, Huang Y, Ding M, Shen Q, Li C J Cell Mol Med. 2021; 26(3):750-763.

PMID: 34935260 PMC: 8817121. DOI: 10.1111/jcmm.17110.

Absence of PARP-1 affects Cxcl12 expression by increasing DNA demethylation.

Tolic A, Grdovic N, Dinic S, Rajic J, Dordevic M, Sinadinovic M J Cell Mol Med. 2019; 23(4):2610-2618.

PMID: 30697918 PMC: 6433732. DOI: 10.1111/jcmm.14154.

CXCL12 protects pancreatic β-cells from oxidative stress by a Nrf2-induced increase in catalase expression and activity.

Dinic S, Grdovic N, Uskokovic A, Dordevic M, Mihailovic M, Arambasic Jovanovic J Proc Jpn Acad Ser B Phys Biol Sci. 2016; 92(9):436-454.

PMID: 27840391 PMC: 5328787. DOI: 10.2183/pjab.92.436.

The Importance of the CXCL12/CXCR4 Axis in Therapeutic Approaches to Diabetes Mellitus Attenuation.

Vidakovic M, Grdovic N, Dinic S, Mihailovic M, Uskokovic A, Arambasic Jovanovic J Front Immunol. 2015; 6:403.

PMID: 26300887 PMC: 4528295. DOI: 10.3389/fimmu.2015.00403.

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