Spatial Proteomic and Phospho-proteomic Organization in Three Prototypical Cell Migration Modes

Overview

Journal Proteome Sci

Publisher Biomed Central

Date 2014 Jul 3

PMID 24987309

Citations 1

Authors

Georgios Fengos

Alexander Schmidt

Katrin Martin

Erika Fluri

Ruedi Aebersold

Dagmar Iber

Olivier Pertz

Affiliations

Soon will be listed here.

Abstract

Background: Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for localized membrane protrusion that drives directed cell migration. Different ensembles of proteins are therefore likely to get recruited and phosphorylated in membrane protrusions in response to specific cues.

Results: HERE, WE USE AN ASSAY THAT ALLOWS TO BIOCHEMICALLY PURIFY EXTENDING PROTRUSIONS OF CELLS MIGRATING IN RESPONSE TO THREE PROTOTYPICAL RECEPTORS: integrins, recepor tyrosine kinases and G-coupled protein receptors. Using quantitative proteomics and phospho-proteomics approaches, we provide evidence for the existence of cue-specific, spatially distinct protein networks in the different cell migration modes.

Conclusions: The integrated analysis of the large-scale experimental data with protein information from databases allows us to understand some emergent properties of spatial regulation of signaling during cell migration. This provides the cell migration community with a large-scale view of the distribution of proteins and phospho-proteins regulating directed cell migration.

Citing Articles

Proteome Based Construction of the Lymphocyte Function-Associated Antigen 1 (LFA-1) Interactome in Human Dendritic Cells.

Eich C, Lasonder E, Cruz L, Reinieren-Beeren I, Cambi A, Figdor C PLoS One. 2016; 11(2):e0149637.

PMID: 26889827 PMC: 4758637. DOI: 10.1371/journal.pone.0149637.

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