Phosphatidylethanolamine Biomimetic Coating Increases Mesenchymal Stem Cell Osteoblastogenesis

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2014 Jul 2

PMID 24980874

Citations 5

Authors

Berengere J C Luthringer

Uma M R Katha

Regine Willumeit

Affiliations

Soon will be listed here.

Abstract

Previous observations (e.g., decreased bacterial adhesion) have shed the light on the auspicious possibility to use phosphatidylethanolamine as biomimetic coating for metal implants. Additionally, it was experimentally shown that phosphatidylethanolamine induces bone formation, however, up to now no study was performed to understand this observation or to find an explanation. In an attempt to unveil how and why phosphatidylethanolamine can improve cell metabolism and osteogenic differentiation, primary cells (human umbilical cord perivascular cells) were cultured on native or phosphatidylethanolamine coated surfaces. Several parameters were followed on gene (real time polymerase chain reaction) and protein (e.g., dot-blot and ELISA tests) levels. It was determined that phosphatidylethanolamine potentiates cell metabolism, osteogenic differentiation, and mineralisation early processes. By preventing biofilm formation while promoting new bone formation, phosphatidylethanolamine could be easily implemented as implant bio-mimicking coating.

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References

Schlegel R, Williamson P . Phosphatidylserine, a death knell. Cell Death Differ. 2001; 8(6):551-63. DOI: 10.1038/sj.cdd.4400817. View

van Tuyl L, Voskuyl A, Boers M, Geusens P, Landewe R, Dijkmans B . Baseline RANKL:OPG ratio and markers of bone and cartilage degradation predict annual radiological progression over 11 years in rheumatoid arthritis. Ann Rheum Dis. 2010; 69(9):1623-8. DOI: 10.1136/ard.2009.121764. View

Santin M, Rhys-Williams W, OReilly J, Davies M, Shakesheff K, Love W . Calcium-binding phospholipids as a coating material for implant osteointegration. J R Soc Interface. 2006; 3(7):277-81. PMC: 1578739. DOI: 10.1098/rsif.2005.0088. View

Willumeit R, Schossig M, Clemens H, Feyerabend F . In-vitro interactions of human chondrocytes and mesenchymal stem cells, and of mouse macrophages with phospholipid-covered metallic implant materials. Eur Cell Mater. 2007; 13:11-25. DOI: 10.22203/ecm.v013a02. View

Takuwa Y, Ohse C, Wang E, Wozney J, Yamashita K . Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3-E1. Biochem Biophys Res Commun. 1991; 174(1):96-101. DOI: 10.1016/0006-291x(91)90490-x. View

Willumeit R, Schuster A, Iliev P, Linser S, Feyerabend F . Phospholipids as implant coatings. J Mater Sci Mater Med. 2007; 18(2):367-80. DOI: 10.1007/s10856-006-0702-9. View

Eanes E . Mixed phospholipid liposome calcification. Bone Miner. 1992; 17(2):269-72. DOI: 10.1016/0169-6009(92)90749-4. View

Bialek P, Kern B, Yang X, Schrock M, Sosic D, Hong N . A twist code determines the onset of osteoblast differentiation. Dev Cell. 2004; 6(3):423-35. DOI: 10.1016/s1534-5807(04)00058-9. View

Samee N, Geoffroy V, Marty C, Schiltz C, Vieux-Rochas M, Levi G . Dlx5, a positive regulator of osteoblastogenesis, is essential for osteoblast-osteoclast coupling. Am J Pathol. 2008; 173(3):773-80. PMC: 2527089. DOI: 10.2353/ajpath.2008.080243. View

10.

Hall B, Miyake T . Divide, accumulate, differentiate: cell condensation in skeletal development revisited. Int J Dev Biol. 1995; 39(6):881-93. View

11.

Chenu C, Pfeilschifter J, Mundy G, Roodman G . Transforming growth factor beta inhibits formation of osteoclast-like cells in long-term human marrow cultures. Proc Natl Acad Sci U S A. 1988; 85(15):5683-7. PMC: 281824. DOI: 10.1073/pnas.85.15.5683. View

12.

Crockett J, Rogers M, Coxon F, Hocking L, Helfrich M . Bone remodelling at a glance. J Cell Sci. 2011; 124(Pt 7):991-8. DOI: 10.1242/jcs.063032. View

13.

Lefever S, Vandesompele J, Speleman F, Pattyn F . RTPrimerDB: the portal for real-time PCR primers and probes. Nucleic Acids Res. 2008; 37(Database issue):D942-5. PMC: 2686610. DOI: 10.1093/nar/gkn777. View

14.

Letellier S, Lochhead M, Campbell A, Vogel V . Oriented growth of calcium oxalate monohydrate crystals beneath phospholipid monolayers. Biochim Biophys Acta. 1998; 1380(1):31-45. DOI: 10.1016/s0304-4165(97)00121-9. View

15.

Krishna O, Kim K, Byun Y . Covalently grafted phospholipid monolayer on silicone catheter surface for reduction in platelet adhesion. Biomaterials. 2005; 26(34):7115-23. DOI: 10.1016/j.biomaterials.2005.05.023. View

16.

Olivares-Navarrete R, Sutha K, Hyzy S, Hutton D, Schwartz Z, McDevitt T . Osteogenic differentiation of stem cells alters vitamin D receptor expression. Stem Cells Dev. 2011; 21(10):1726-35. PMC: 3376455. DOI: 10.1089/scd.2011.0411. View

17.

Lampasso J, Marzec N, Margarone 3rd J, Dziak R . Role of protein kinase C alpha in primary human osteoblast proliferation. J Bone Miner Res. 2002; 17(11):1968-76. DOI: 10.1359/jbmr.2002.17.11.1968. View

18.

Boguslawski G, Hale L, Yu X, Miles R, Onyia J, Santerre R . Activation of osteocalcin transcription involves interaction of protein kinase A- and protein kinase C-dependent pathways. J Biol Chem. 2000; 275(2):999-1006. DOI: 10.1074/jbc.275.2.999. View

19.

Kochanowski A, Hoene A, Patrzyk M, Walschus U, Finke B, Luthringer B . Examination of the inflammatory response following implantation of titanium plates coated with phospholipids in rats. J Mater Sci Mater Med. 2011; 22(4):1015-26. DOI: 10.1007/s10856-011-4287-6. View

20.

Luthringer B, Ali F, Akaichi H, Feyerabend F, Ebel T, Willumeit R . Production, characterisation, and cytocompatibility of porous titanium-based particulate scaffolds. J Mater Sci Mater Med. 2013; 24(10):2337-58. DOI: 10.1007/s10856-013-4989-z. View